摘要
双钩钻为常用瑶药,但是目前对其研究比较少,为开发利用其资源,采用HPLC法同时测定其中含有的去氢钩藤碱、异钩藤碱、异去氢钩藤碱、钩藤碱、缝籽嗪甲醚、去氢毛钩藤碱和毛钩藤碱等7种生物碱类成分含量,确认其入药的科学性。结果表明,7种成分在各自范围内线性关系良好(r≥0.9991),平均加样回收率为97.37%~103.29%,RSD为1.40%~3.02%。不同产地双钩钻中均含有7种生物碱类成分,以缝籽嗪甲醚和去氢毛钩藤碱的含量较高。本法简便易行,重现性好,为双钩钻的质量控制及资源开发应用提供了科学依据。
As a common yao medicine,but there are few studies on Uncariae Radix.For the development and utilization of Uncariae Radix resources,the HPLC method was established to determine the content of seven components in it to confirm the scientific nature of its application.The results showed that seven constituents showed good linear relationships within their own ranges(r≥0.9991),whose average recoveries were 97.37%~103.29%with the RSDs of 1.40%~3.02%.There were 7 alkaloids in the Uncariae Radix from different producing areas,and the contents of geissoschizine methylether、hirsuteine were higher.This method is simple,easy to operate and has good reproducibility,which provides a scientific basis for the resource development,application and quality control of Uncariae Radix.
作者
谢锋
蓝相
林青华
蒋林
Xie Feng;Lan Xiang;Lin Qinghua;Jiang Lin(Guangxi university of traditional Chinese medicine,Nanning 530200;Guangxi Zhuang Yao Pharmaceutical Engineering technology Research Center,Nanning 530200)
出处
《壮瑶药研究》
2023年第1期102-105,311,共5页
Research of Zhuang and Yao Ethnic Medicine
基金
广西壮瑶药工程技术研究中心开放课题(KJT1900107)
广西壮瑶药重点实验室(桂科基字[2014]32号)
壮瑶药协同创新中心(桂教科研[2013]20号)
广西壮族自治区民族药资源与应用工程研究中心(桂发改高技函[2020]2605号)
广西中医药重点学科壮药学(GZXK-Z-20-64)
关键词
瑶药
双钩钻
生物碱
含量测定
Yao medicine
Uncariae Radix
alkaloid
Content determination
