摘要
建立针叶樱桃提取物中芦丁含量的高效液相色谱测定方法。针叶樱桃提取物样品用超声波辅助提取法处理后,进行高效液相色谱分析,色谱柱为Diamonsil C18(200×4. 6mm,5 (m),以乙腈-0. 1%冰醋酸为流动相进行线性梯度洗脱,总流速为1. 0mL·min^(-1),柱温30℃,检测波长360nm,进样量20μL。研究结果表明,芦丁在1. 180μg·mL^(-1)～11. 80μg·mL^(-1)范围内线性关系良好(r=0. 9988),平均加样回收率为94. 5%,RSD均小于2. 0%,回收率符合要求,所建立方法快速、简便,能够用于测定针叶樱桃提取物中芦丁的含量。
To develop a method for the determination of rutin in extract of Acerola cherry.An HPLC method has been developed to determine rutin on Agilent C18(4.6mm×250mm,5(m)column with the mobile phase of consisting of acetonitrile and 0.1%acetic acid.The UV detection wavelength was set at 360nm.The result showed that linearity of rutin was good in the range of 1.180-11.80μg·mL^-1(r=0.9988).The average recovery of rutin was 94.5%with RSD less than 2.0%.The method is sample and accurate and could be used to control the quality of Acerola cherry.
作者
周朋
石美丽
周倩
林凤辉
秦晋颖
刘志刚
Zhou Peng;Shi Mei-li;Zhou Qian;Lin Feng-hui;Qin Jin-yin;Liu Zhi-gang(Food and Pharmaceutical Engineering, Guiyang University, Guizhou Guiyang 550005, China)
出处
《贵阳学院学报（自然科学版）》
2018年第4期60-61,65,共3页
Journal of Guiyang University：Natural Sciences
基金
2016年贵州省高校大学生创新创业训练计划资助
