摘要
采用超声波辅助技术对槐米(国槐花蕾)芦丁进行提取,获得最佳工艺参数:20℃,超声波频率20kHz、功率800W,料液比1∶15,提取时间10min,重复2次。利用分子形态转变法进一步进行纯化,并采用高效液相色谱技术(HPLC)测定了芦丁的纯度。将槐米芦丁与金属铬(Cr3+)进行配合,通过原子吸收光谱(AAS)、紫外光谱(UV)、红外光谱(IR)对槐米芦丁铬(Cr3+)配合物分子结构进行分析,结果表明:槐米芦丁与铬(Cr3+)按摩尔比1∶2的比例反应生成槐米芦丁铬(Cr3+)配合物;其中槐米芦丁分子中的5位羟基、4位羰基,B环中的2个苯环邻羟基均参与了配合反应。
The extraction of rutin from Sophora japonica L. flower buds by ultrasonic assistance technology was investigated, and the optimum conditions were obtained as follows： ultrasonic frequency and power were 20 kHz and 800 W, ratio of material to fluid was 1： 15, and the extraction was carried out at 20 ℃ for 10 min and repeated twice. The extracted rutin was further purified by the method of changing molecular formation, and its purity was determined by HPLC. With AAS, UV spectra and IR spectra, the structure of the complex of rutin with chromium （Cr3 ＋ ） were studied. The results showed that the molar ratio of rutin to chromium （Cr3＋ ） was 1 ： 2. Meanwhile, 5-hydroxyl group, 4-carbonyl group and orthodihydroxyphenyl in ring B were found to have taken part in the coordination reaction with metal ions Cr3 ＋.
出处
《北京林业大学学报》
CAS
CSCD
北大核心
2011年第1期144-149,共6页
Journal of Beijing Forestry University
基金
中华人民共和国环境保护总局项目(2008020401)
关键词
槐米
芦丁
提取
纯化
芦丁铬
光谱分析
Sophora japonica L. flower buds
rutin
extraction
purification
complex of rutin with chromium （ Cr3 ＋ ）
spectral analysis
