摘要
邻苯二甲酸酯(PAEs)是环境中普遍存在的有机污染物,具有环境雌激素效应,对人体健康和生态安全造成严重威胁。菌株YC-JY1分离自受石油长期污染的土壤中,可以利用邻苯二甲酸二丁酯(DBP)作为唯一的碳源进行生长;经16S rDNA鉴定,确定其属于黄色杆菌属(Xanthobacter sp.)。菌株YC-JY1降解DBP的最适条件为30℃,pH 7.0,无NaCl添加;在此条件下,100mg/L DBP在5 d内能被完全降解。随着DBP浓度的升高,菌株YC-JY1在5 d内对200 mg/L-400 mg/L DBP的降解率在94%以上;通过底物谱实验发现,菌株YC-JY1对其它邻苯二甲酸酯具有广泛的利用能力,其中邻苯二甲酸二戊酯(DPeP),邻苯二甲酸二己酯(DHP)的降解率均在90%以上;通过高效液相色谱-质谱联用确定DBP中间代谢产物为邻苯二甲酸单丁酯(MBP),邻苯二甲酸(PA)。由此推断,在菌株YC-JY1的作用下,DBP的起始代谢途径为DBP首先水解为MBP,继而水解为PA。
Phthalic acid esters(PAEs)are ubiquitous organic pollutants with environmental estrogen effects,which cause serious threats to human health and safety of ecological system.Strain YC-JY1 was isolated from long-time contaminated soil by petroleum,and grew by utilizing dibutyl phthalate(DBP)as the sole carbon source.After 16S rDNA sequencing,it was identified to be a strain of Xanthobacter genus.The optimal conditions for DBP degradation by this strain were 30℃,pH 7.0 and no NaCl added.Under this condition,100 mg/L DBP was completely degraded within 5 d.As the concentration of DBP increased,the degradation rate of it to DBP with initial concentration of 200-400 mg/L was over 94%after 5 d.Through substrate spectrum experiments,it was found that strain YC-JY1 utilized extensively other PAEs.The degradation rate of it to dipentyl phthalate(DPeP)and dihexyl phthalate(DHP)were all above 90%.The intermediates of DBP were determined to be mono-butyl phthalate(MBP)and phthalic acid(PA)by HPLC-MS.It was inferred that the initial metabolic process of DBP was as firstly hydrolyzed to MBP and then to PA by the strain YC-JY1.
作者
王嘉翼
樊双虎
任超
王俊欢
杨婷
贾阳
李先军
闫艳春
WANG Jia-yi;FAN Shuang-hu;REN Chao;WANG Jun-huan;YANG Ting;JIA Yang;LI Xian-jun;YAN Yan-chun(Graduate School of Chinese Academy of Agricultural Sciences,Beijing 100081)
出处
《生物技术通报》
CAS
CSCD
北大核心
2018年第10期157-164,共8页
Biotechnology Bulletin
基金
国家自然科学基金项目(31170119
31540067)
中国农业科学院基本科研业务费(0042014011
1610042017001)
