摘要
为挖掘毒死蜱降解内生菌资源、强化功能内生菌在农业生产中的应用,通过微生物梯度驯化技术,从农药池周边生长的水稻根组织中分离纯化获得一株能以毒死蜱为唯一碳源的降解内生菌CP40,研究了该菌株的毒死蜱降解特性及对作物中残留毒死蜱降解的影响。基于16S rRNA和细菌形态学特征,将菌株CP40鉴定为芽孢杆菌(Bacillus sp.CP40)。菌株CP40含有机磷降解酶基因opd,异源表达该基因后毒死蜱的降解率达58.4%。降解条件优化实验表明,毒死蜱初始浓度为10 mg·L^(-1)、温度为30℃、pH为7时,菌株CP40对毒死蜱的降解效果最佳。此外,发现菌株CP40具有促生能力,接种菌株CP40可显著促进水稻生长,并能将水稻体内毒死蜱含量降低30.9%。研究表明,功能内生菌在调控水稻体内毒死蜱的降解过程中可发挥重要作用。
To tap the resources of chlorpyrifos-degrading endophytic bacteria and apply these bacteria in bioremediation to improve agricultural production,a chlorpyrifos-degrading endophyte strain was isolated from the roots of rice(Oryza sativa L.)using a microbiological culture method in a contaminated factory.The characteristics and effect of the strain on chlorpyrifos degradation in rice crops were investigated.Based on the morphological characteristics of the endophyte and 16S rRNA sequence analysis,the strain was identified as Bacillus sp.and named as CP40.Bacillus sp.CP40 contained opd,which encodes an organophosphorus hydrolase.The degradation rate of chlorpyrifos was 58.4% when the gene was heterologously expressed.Optimization analysis of the degradation conditions indicated that the lowest chlorpyrifos residues were observed at an initial chlorpyrifos concentration of 10 mg·L^(-1),temperature of 30℃,and pH of 7.Strain CP40 colonized and promoted the growth and development of rice plants and removed 30.9% of chlorpyrifos from these plants.Our results suggest that endophytic bacteria can reduce chlorpyrifos residues in rice.
作者
卢映菲
马丽雅
张召贤
孙星
葛静
吴祥为
余向阳
LU Yingfei;MA Liya;ZHANG Zhaoxian;SUN Xing;GE Jing;WU Xiangwei;YU Xiangyang(College of Resources and Environment,Anhui Agricultural University,Hefei 230036,China;Institute of Food Safety and Nutrition,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China)
出处
《农业环境科学学报》
CAS
CSCD
北大核心
2022年第12期2763-2771,共9页
Journal of Agro-Environment Science
基金
国家自然科学基金项目(32272600)
江苏省农业科技自主创新资金项目(CX(22)3074)
国家重点研发计划项目(2021YFD1700803)。
关键词
毒死蜱
内生菌
降解特性
定殖
水稻
chlorpyrifos
endophyte
degrading characteristic
colonization
rice
