摘要
目的:探讨高效液相色谱法(HPLC)同时测定百草枯、高效氯氟氰菊酯含量的研究。方法:采用Agilent HC-C18柱(4.6 mm×150 mm,5μm),流动相为乙腈-水(90∶10),流速为1.5 mL·min-1,VWD紫外检测器,检测波长230 nm,柱温30℃。结果:百草枯的保留时间约为1.048 min,高效氯氟氰菊酯的保留时间约为2.618 min。百草枯在0.75~2.00 mg·mL^(-1)的范围内具有良好的线性关系(r=0.9995),平均加样回收率为100.04%,RSD为1.44%,检测限为0.625μg·mL^(-1);高效氯氟氰菊酯在0.10~1.00 mg·mL^(-1)范围内有良好的线性关系(r=0.9997),平均加样回收率为100.32%,RSD为1.26%,检测限为0.08μg·mL^(-1)。结论:HPLC快速、准确、重复性好,适用于同时测定百草枯和高效氯氟氰菊酯,可用于临床农药中毒急诊诊断。
Objective:To establish a method for simultaneous determination of paraquat and lambda-cyhalothrin by high performance liquid chromatography(HPLC).Methods:Agilent HC-C18column(4.6mm×150mm,5μm),mobile phase of acetonitrile water(90∶10),flow rate of1.5mL·min-1,VWD UV detector,detection wavelength of230nm,column temperature of30℃.Results:The retention time of paraquat was about1.048min,and the retention time of lambda-cyhalothrin was about2.618min.The paraquat in the range of0.75~2.00mg·mL-1had a good linear relationship(r=0.9995),the average recovery was100.04%,RSD was1.44%,the detection limit is0.625μg·mL-1.Lambda-cyhalothrin in the range of0.10~1.00mg·mL-1had a good linear relationship(r=0.9997),the average recovery rate was100.32%,RSD was1.26%,the detection limit is0.08μg·mL-1.Conclusion:HPLC method is rapid,accurate and reproducible.It is suitable for simultaneous determination of paraquat and lambda-cyhalothrin.It can be used for emergency diagnosis of clinical pesticide poisoning.
作者
夏军龙
王毅刚
王景景
王璐
田研
郭倩
XIA Junlong;WANG Yigang;WANG Jingjing;WANG Lu;TIAN Yan;GUO Qian(Department of Clinical Pharmacy,215 Hospital of Shaanxi Nuclear Industry,Shaanxi Xianyang712000,China)
出处
《中国医药导刊》
2017年第11期1230-1233,共4页
Chinese Journal of Medicinal Guide
基金
陕西省核工业地质局科研项目(项目编号:2012C05
项目名称:药学实验方法在农药中毒解救过程中的基础应用研究)
