摘要
目的比较不同比例激活剂对富血小板凝胶的生物学影响,研究其用于角膜基质再生的可行性。方法抽取兔全血,经二次离心法制备富血小板血浆(PRP),将PRP与激活剂按不同比例激活,制备PRP凝胶。实验分为3个实验组,分别为9∶1组(PRP∶激活剂=9∶1)、11∶1组(PRP∶激活剂=11∶1)及5∶1组(PRP∶激活剂=5∶1)。将兔角膜基质细胞与PRP凝胶共同培养,进行组织形态学观察,用细胞增殖-毒性检测试剂盒法,分别检测3个实验组PRP凝胶释放的复合生长因子促角膜基质细胞的增殖作用。对不同实验组制备的PRP凝胶与双层猪角膜基质进行黏附性观察。结果 9∶1组制备的PRP凝胶促进兔角膜基质细胞的增殖和活化作用强于其他实验组。3个实验组中,只有9∶1组制备的PRP凝胶苏木精-伊红染色法染色结果显示,呈有序的网状支架结构,能紧密黏附并固定双层猪角膜基质。结论 PRP与激活剂以9∶1比例制备的PRP凝胶促角膜基质细胞增殖作用最强,同时具有有序网状结构和良好的组织黏附性及相容性,是一种可以用于角膜基质再生的良好支架。
Objective To compare the effect of different proportions of activators on the biological functionof platelet-rich gel and to reaserch the feasibility for the reconstruction of corneal stroma.Methods Rabbitblood was extracted and platelet-rich plasma(PRP)was prepared by two-step centrifugation.Platelet-rich gelswere prepared by activation of PRP with thrombin activators in different proportions and divided into threegroups.There were group9:1(PRP:activators=9:1),group11:1(PRP:activators=11:1)and group5:1(PRP:activators=5:1).The rabbit keratocytes were seperately co-cultured with the three platelet-rich gelsand the morphological features were observed.CCK-8assay was used to analyze the influences of compositegrowth factors released from the platelet-rich gel on the proliferation of rabbit keratocytes in the three groups.The adhesive properties of the three platelet-rich gels were evaluated after adhesion to the double-layerporcine corneal stroma.Results The platelet-rich gel of the group9:1had better effect on promoting cellproliferation than those of other two groups.HE staining displayed that only the platelet-rich gel prepared inthe group9:1formed an orderly network scaffold that could closely attach to the double-layer porcine cornealstroma.Conclusions The platelet-rich gel,prepared by combining PRP with activators in9:1proportion,can effectively promote the proliferation of keratocytes,and has the nature of orderly network structure,effectiveadhesiveness and favorable biocompatibility.These properties indicate that the platelet-rich gel prepared bycombining PRP with activators in9:1proportion is an excellent scaffold for the reconstruction of cornealstroma.
作者
戴静
陈建苏
招志毅
Jing Dai;Jian-su Chen;Zhi-yi Zhao(Department of Ophthalmology, Liuzhou Worker's Hospital, Liuzhou, Guangxi 545005, China;Research Room of Ophthalmology, Key Laboratory of Regenerative Medicine of Ministry of Education, Jinan University, Guangzhou, Guangdong 510632, China)
出处
《中国现代医学杂志》
CAS
北大核心
2017年第20期16-20,共5页
China Journal of Modern Medicine
基金
国家自然科学基金(No:30973244)
