摘要
目的:富含血小板血浆中含有较高浓度的生长因子,对骨的再生和修复都有一定的促进作用。通过大体和扫描电镜两种方法观察富含血小板血浆凝胶的结构,探索其用于组织工程载体的可能性。方法:实验于2004-01/03在第四军医大学口腔医学院动物中心完成。①制备牛凝血酶溶液:作为使富含血小板血浆结固成凝胶的催化剂。②制备富含血小板血浆液:将清洁级新西兰兔4只,解剖颈内静脉,抽取静脉血10mL,离心后将红细胞与血浆分离,再将血浆离心,使富含血小板血浆与乏血小板血浆分开,得到富含血小板血浆。③制备富含血小板血浆凝胶:将富含血小板血浆与牛凝血酶溶液按体积比6∶1充分混合为凝胶。④冻干及观察:将富含血小板血浆凝胶冻干,进行大体观察和扫描电子显微镜检查。结果:①富含血小板血浆凝胶大体观察结果:可见大量的纤维彼此细密交织。②富含血小板血浆凝胶扫描电子显微镜观察结果:材料内大量的纤维交织,形成支架,其间含有大量的孔隙,孔隙彼此相通,孔径为200～1000μm,孔隙率80%。结论:富含血小板血浆凝胶呈典型的多孔性结构,孔径大小、孔隙率均比较符合骨组织工程支架材料的要求,而且又含有丰富的、对成骨有利的生长因子,提示富含血小板血浆可用作骨组织工程支架材料。
AIM： There are growth factors in a high concentration in the platelet-rich plasma, which can improve bone regeneration and repair. To observe the structure of platelet-rich plasma gel by gross observation and scanning electron microcopy so as to estimate the possibility of it used as a scaffold of tissue engineering. METHODS： The experiment was conducted in the Center for Experimental Animals,Stomatological College,Fourth Military Medical University from January to March 2004. ①Bovine thrombin solution was prepared to be used as the catalyst of platelet-rich plasma into gel. ② Preparation of platelet-rich plasma solution： Internal jugular vein was dissected from 4 New Zealand rabbits in clean grade to phlebotomize 10 mL venous blood. Then, plasma and red cells were separated after centrifugation, followed by centrifugation of plasma to separate the plateletrich plasma and platelet-lack plasma. ③Platelet-rich plasma gel was prepared by compounding platelet-rich plasma and bovine thrombin solution in the volume ratio of 6 to 1. ④The prepared platelet-rich plasma gel was lyophilized, and then was observed grossly and by scanning electron microscopy. RESULTS： ①Platelet-rich plasma gel was interweaved each other thickly by abundant fibers through gross observation. ②By observation under a scanning electron microscope： Lots of fibers were interweaved in plateletrich plasma gel and formed a scaffold, among which many interconnecting wells with the ostiole from 200 to 1000 μm were found in the porosity of 80%. CONCLUSION： Attributing to its typical polyporous structure, ostioie size and porosity suitable for the scaffold of tissue engineering, as well as plenty of growth factors beneficial to osteogenesis, platelet-rich plasma gel can be used as the material for scaffold of tissue engineering.
出处
《中国临床康复》
CSCD
北大核心
2005年第26期114-115,i0005,共3页
Chinese Journal of Clinical Rehabilitation
