摘要
目的探讨不同条件下胚胎脊髓移值修复成鼠急性脊髓损伤(SCI)的能力。方法成鼠胸 髓损伤后分别移植孕14d胚胎脊髓(FSC组)、游离正中神经加胚胎脊髓(P＋F组)、带血管蒂正 中神经加胚胎脊髓(V＋F组)，术后8周行组织学检查。结果V＋F组胚胎脊髓与受体融合佳， 体积增长速度、神经纤维和神经元数目显著高于P＋F、FSC组(P< 0.01)，细胞分化较好，突 触较成熟，界面区也无明显的胶质增生。结论带血管蒂周围神经与胚胎脊髓联合移植，对FS C的生长发育、对损伤神经元的再生能力均有一定的促进作用。
Objective To observe the repair effect of fetal spinal cord in differen t conditions.Method A 5 mm cord defect of the left lateral column was made at T1 －3 vertebral level, and then the defect was grafted randomly using embryonic da y 14 fetal spinal cord (Group FSC), free peripheral nerve and fetal spinal cord (Group P＋F), or vascularized peripheral nerve and fetal spinal cord (Group V＋F ). 8 weeks after surgery, the survival, differentiation of the grafts, and the a bility of repairing host spinal cord were assessed.Result In VPN－FSC co－graft group, the fetal implant was intimately fused with the host CNS parenchyma, the volume, nerve fibers and neurons density of the fetal implant were significantly higher than Group FSC and Group P＋F(P< 0.01). The majority of neurons in this group were well differentiated, well－developed synapses were scattered througho ut the neuropil.Conclusion The combination of VPN－FSC could prompt the developm ent of fetal implant and support the regeneration of central injured axons to ce rtain degrees.
出处
《中国组织工程研究》
CAS
CSCD
2001年第8期50-51,共2页
Chinese Journal of Tissue Engineering Research
关键词
胚胎脊髓
移植
脊髓损伤
fetal spinal cord
graft
spinal cord injury
