摘要
背景:近年来不断报道用自体周围神经植入治疗脊髓损伤的研究,但功能恢复尚不满意。目的:探讨自体肋间神经不同移植术式对治疗脊髓损伤疗效的影响。设计:随机对照的实验研究。地点和对象:在苏州大学附属第二医院实验中心完成。SD大鼠60只,来自苏州大学医学院实验动物中心,体质量176～291g。方法:采用SD鼠,经T8,T9平面切除4mm长的半侧脊髓制成脊髓半切模型。分3组,分别用6～8条纤细的肋间神经节段按近端白质-远端灰质和近端白质-远端白质两种桥接术式填充缺损处,对照组用明胶海绵填充脊髓半切残腔。饲养4～6周,观察结果。主要观察指标:动物活动能力Tarlov评分,斜板试验,组织学观察。结果:两种术式受试鼠的后肢功能部分恢复,术后6周,A,B组Tarlov评分分别为(2.0±1.58),(2.1±1.20)分,均高于对照组,斜板试验临界角度分别为(42.2±2.94)°,(42.1±1.46)°,均较对照组明显提高;镜下见移植组织存活、充填于缺损处,部分与脊髓组织融合;组织、功能的恢复均明显优于对照组,但两种术式组间无明显差别。结论:脊髓半切模型可能是研究周围神经植入的合适模型,肋间神经可能是适合的周围神经植入材料,周围神经的桥接术式有待进一步研究。
BACKGROUND:Recently,a lot of studies were reported on repair of injured spinal cord by transplanting autologous peripheral nerve.However,the functional recovery is not very satisfactory. OBJECTIVE:To explore the effects of different transplantation methods for autologous intercostal nerves on spinal cord injury(SCI). DESIGN:A randomized controlled experimental study. SETTING and PARTICIPANTS:The study was completed in the Laboratory of Second Hospital Affiliated to Suzhou University.Sixty SD rats,weighing 176-291 g,were provided by Center for Experimental Animals of Suzhou University. METHODS:SD rats were used to make spinal cord hemisection model by cutting a 4 mm length one side of spinal cord from T8 and T9 and then divided into three groups.6 to 8 tenuous intercostal nerve segments were used in the first group to fill the defect area by means of proximal white matter linking to distal part gray matter,while in the way of proximal white matter linking to distal white matter in the second group.The third group was used gelfoam to fill the defect as control group.Results were observed after 4 to 6 weeks of feeding. MAIN OUTCOME MEASURES:Tarlov scoring of animal activities;gradient plate test;histological observation. RESULTS:The functions of posterior limbs of rats in two experimental groups were partly restored.Six weeks after operation,the Tarlov scores of two groups were(2.0±1.58) and (2.1±1.20) respectively which were all higher than that of control group.The critical angles of gradient plate test in two groups were(42.2±2.94)°and (42.1±1.46)°respectively,bigger than that in control group.It was found that transplanted tissue survived and filled into defect and partly fused with spinal cord.The recoveries of tissue and function were all better than that of control group.However,there was no difference between two experimental groups. CONCLUSION:Spinal cord hemisection model may be the appropriate model to study the transplantation of peripheral nerves,while the intercostals nerves may be the s
出处
《中国临床康复》
CSCD
2004年第32期7313-7315,共3页
Chinese Journal of Clinical Rehabilitation
