摘要
目的证实体外培养SD大鼠破骨细胞存在凋亡现象，并探讨其凋亡的规律。方法体外培养SD大鼠破骨细胞，采用高倍显微镜下形态学观察法和DNA 3－OH末端缺口原位标记法(TUNEL法)标记凋亡细胞，并推测其凋亡的规律。结果体外培养的破骨细胞存在凋亡现象，凋亡破骨细胞的数目和比例随着培养时间的延长而逐渐增加，在培养的第3、4、5、6天其凋亡率分别为10％、14％、34％、50％。结论体外培养的破骨细胞存在凋亡现象，随着培养时间的延长凋亡率逐渐增加。
Objective To study the phenomena and the law of apoptosis about osteoclasts of SD rat cultured in vitro. Method The phenomena about apoptosis of osteoclasts of SD rat cultured in vitro were investigated through morpholog and DNA 3－OH terminal deoxynucleotidyl transferase－mediated deoxyuride tiphosphate nick－end labeling by microscope, and the law of apoptosis was suspected. Result The phenomena of apoptosis was observed, the number and the ratio of osteoclasts in apoptosis were increasing by the time of culture. The ratio of apoptosis of osteoclasts were 10, 14, 34 and 50 percent at the date of 3, 4, 5 and 6 respectively. Conclusion Apoptosis is existed in osteoclasts of SD rat cultured in vitro, the ratio of apoptosis of osteoclasts is increasing by the time of culture.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
2001年第20期46-47,共2页
Journal of Clinical Rehabilitative Tissue Engineering Research
关键词
破骨细胞
凋亡
骨质疏松
osteoclast
apoptosis
osteoporosis
