摘要
以筛选出的长叶点地梅(Androsace longifolia)淡绿色疏松的愈伤组织为试验材料,比较了不同激素、蔗糖浓度、摇床转速等对细胞生长的影响,测定了培养过程中培养液pH值、电导率及干质量等变化。结果表明,长叶点地梅愈伤细胞悬浮培养的最佳激素为6-BA,浓度为0.3 mg·L-1,细胞干质量达0.150 g。愈伤组织接种量为1 g时,细胞干质量较高,最佳蔗糖浓度为2%,最佳摇床转速为105 r·min-1。在悬浮培养过程中,培养液的pH呈先下降后缓慢上升的变化趋势,在培养21 d时基本趋于稳定;随着细胞数量的增加,溶液的电导率呈先下降后上升的趋势,在15 d时,达到最低,根据细胞干质量的测定,筛选出悬浮培养细胞的最佳收获时间为12 d。
The light green loose callus of Androsace longifolia were selected as the basic experimental materials.The effects of different hormones,sucrose concentrations,and shaker speeds on the cell dry weight on cell suspension cultures were compared. The results showed that the optimal hormone of A. longifolia suspension culture was 6-BA which had cell dry weight of 0. 15 g with optimal concentration of 0. 3 mg·L- 1. Cell dry weight was higher when the inoculation amount of the callus was 1 g. The optimal sucrose concentration and shaker speed was 2%and 105 r ·min- 1,respectively. During the culturing process,the pH value decreased and then gradually increased and stabilized at the day 21 of culture. Conductivity of the culture solution declined and then increased with the increase of cell number which was lowest after 15 days culture. The optimal harvest time of the cell suspension cultures was 12 days after culturing based on the cell dry weights.
出处
《草业科学》
CAS
CSCD
北大核心
2014年第8期1481-1486,共6页
Pratacultural Science
基金
中央高校基本科研业务费专项资金项目(DL12CA17
DL12CA11)
关键词
细胞生长
激素
蔗糖
摇床转速
细胞干质量
cell growth
hormone
sucrose
shaker rotating speed
cell dry weight
