摘要
以长叶点地梅(Androsace longifolia)无菌实生苗为试验材料,研究植物生长调节剂、大量元素、琼脂、蔗糖、暗处理等因子对长叶点地梅愈伤组织诱导的影响。结果表明,2,4-D更适合长叶点地梅叶片愈伤的诱导,诱导愈伤组织最适宜的培养基是MS基本培养基附加1.0 mg·L-1 2,4-D+0.2 mg·L-1 6-BA+3%蔗糖+0.8%琼脂,诱导率为95.0%;暗处理不利于长叶点地梅叶片愈伤的诱导。
Taking A ndrosace longifolia sterile seedlings as experimental materials, the effects of plant growth regulator, macroelement, agar, sugar and dark treatment on A ndrosace longifolia blade callus induction was studied. The results showed that 2,4-D better suited to Androsace longifolia blade callus induction, the most suitable medium was MS basic medium supplemented with 1.0 mg· L^-1 2,4-D ＋ 0.2 mg·L^-1 6-BA ＋ 3% sucrose ＋ 0.8% agar, induction rate was 95.0%; dark treatment was not conducive Androsace leaf callus induction.
出处
《天津农业科学》
CAS
2015年第6期131-134,共4页
Tianjin Agricultural Sciences
