摘要
产黄青霉原生质体制备过程中 ,菌丝体最佳酶解条件与原生质体释放最适条件不同 ,菌丝体培养基含有 0 .0 5 % L-天冬酰胺时 ,选取培养 48h的菌丝体 ,在裂解酶 (L ysing enzym e)浓度 15 mg/ ml,0 .7mol/ LKCl作为渗透压稳定剂的条件下酶解 ,可获得大量原生质体 ;同时 ,再生培养基中含有 2 5 mm ol/ L Ca2 +离子、上层琼脂浓度 1.1% 。
Some factors affecting the preparation and regeneration of protoplasts of Penicillium chrysogenum were studied. It was demonstrated that the optimal conditions were different between the enzymolysis of cell wall and the preparation of protoplasts. Optimal conditions for preparation and regeneration of protoplasts of Penicillium chrysogenum were proposed. Maximum amount of protoplasts were released from mycelia when mycelia grew for 48 hours with shaking in a medium containing 0.05% L asparagine incubated at 30℃for 3 hours in a buffer containing 15mg/ml of Lysing enzyme and 0.7mol/L of KCl used as an osmotic stabilizer. Maximum regeneration frequency of protoplasts was obtained when protoplasts were plated on a regeneration agar containing 25 mmol/L of calcium ion and 1.1% agar in the overlaid medium.
出处
《中国抗生素杂志》
CAS
CSCD
北大核心
2001年第4期241-243,310,共4页
Chinese Journal of Antibiotics
