摘要
目的：研究NF-κB基因沉默对人胃癌细胞SGC7901增殖及TFF3、ERK表达的影响。方法：应用脂质体法将靶向NF-κB的干扰RNA（si RNA）转染入胃癌细胞株SGC7901中。采用MTT法和流式细胞术法检测NF-κB基因沉默对SGC7901细胞的增殖抑制率和细胞凋亡情况,运用Real time PCR和Western blot免疫蛋白印迹法检测SGC7901细胞内TFF3、ERK的表达。结果：MTT法显示NF-κB基因沉默组癌细胞抑制率大于空白载体组和对照组（P〈0.01）;流式细胞术法表明NF-κB基因沉默组细胞凋亡比例达12.60%,明显高于空白载体组和对照组（P〈0.01）;RT-PCR和Western Blot检测示,经NF-κB基因沉默后,细胞内NF-κB、TFF3、ERK m RNA及蛋白表达量下降明显。结论：NF-κB基因沉默可抑制胃癌细胞的增殖并促进其凋亡。NF-κB基因作为胃癌治疗的分子靶点可能具有重要意义。
Objective：To study the effects of NF-κB gene silencing on the proliferation and expression of TFF3 and ERK in human gastric cancer cell line SGC7901. Methods：RNA（si RNA）was transfected into gastric cancer cell line SGC7901 by liposome targeting NF-κB. MTT method and flow cytometry were used to detect the proliferation inhibition and apoptosis of NF-κB gene silencing in SGC7901 cells,the m RNA and protein expression level of TFF3,ERK were detected by Real time PCR and Western blot. Results：MTT showed that the inhibition rate of NF-κB gene silencing group was higher than that of the blank control group and the control group（P〈0.01）;Flow cytometry showed that the percentage of apoptosis in the NF-κB gene silencing group was 12.60%,higher than that in the blank control group and the control group（P〈0.01）;RT-PCR and Western Blot detection,The NF-κB,TFF3,ERK m RNA and protein expression in the cells decreased significantly after NF-κB gene silencing. Conclusion：NF-κB gene silencing can inhibit the proliferation of gastric cancer cells and promote its apoptosis,NF-κB gene may be of great significance as a molecular target for the treatment of gastric cancer.
作者
彭鸿
陈国忠
方丽娇
彭振西
黄丽萍
谢卓恩
苏洪佳
PENG Hong;CHEN Guozhong;FANG Lijiao;PENG Zhenxi;HUANG Liping;XIE Zhuoen;SU Hongjia(Guangxi University of Chinese Medicine,Nanning 530001,Guangxi,China;First Affilialed Hospital of Guangxi University of Chinese Medicine,Nanning 530023,Guangxi,China)
出处
《辽宁中医药大学学报》
CAS
2018年第10期92-95,共4页
Journal of Liaoning University of Traditional Chinese Medicine
基金
国家自然科学基金资助项目(81260529)
