摘要
目的模拟自然感染方式建立百日咳小鼠模型,并初步应用于疫苗评价。方法通过对小鼠品系、周龄,感染用菌株、菌液浊度,感染时间等进行比较,确定动物模型建立的相关参数。然后借助该模型比较全细胞百日咳疫苗和无细胞百日咳疫苗免疫后的小鼠肺部百日咳杆菌的清除情况。程序为第0、2、4周免疫,第6周进行呼吸道感染,感染后2 h、3 d、7 d、14 d、21 d取样,对小鼠肺部菌落进行计数。结果在菌液浊度至少为109CFU/m L时,用Tohama I株对4～10周龄BALB/c小鼠雾化感染30 min,小鼠肺部百日咳杆菌分离培养的阳性率可达100%。用于疫苗评价后发现,免疫全细胞百日咳疫苗的小鼠在感染后7 d检测不到肺内的百日咳杆菌,而免疫无细胞百日咳疫苗的小鼠在感染后21 d还可分离出少量百日咳杆菌。结论通过动物发病、肺部细菌分离培养和感染曲线等对动物模型进行分析,证明通过呼吸道感染途径可成功建立百日咳小鼠模型,并且该模型可应用于百日咳疫苗的评价,为百日咳疫苗的有效性评价奠定重要基础。
Objective To develop a mouse model of pertussis by simulating a natural infection,and a preliminary application was carried out in related vaccine evaluation. Methods The relative parameters of animal models were determined by comparing the mouse strains and ages,bacterial strains,turbidity and infection time.The model was then used to compare the clearance of Bordetella pertussis in the lungs of mice immunized with whole-cell pertussis vaccine and acellular pertussis vaccine,immunized on 0,2,4 weeks,and infected on 6 thweek; samples were collected from mice lungs after infection 2 h,3 d,7 d,14 d,21 d,by which bacterial counting was performed. Results When BALB/c mice with 4 to 10-week-old were infected at least 109 CFU/m L of Tohama I stain for 30 minutes,the positive rate of isolation and culture of Bordetella pertussis in mice lungs could reach 100%. After application,the results showed that the viable counts in samples from lungs were not detected by whole-cell pertussis vaccine immunization at day 7 after infection,however,the same counts were still detectable at day 21 by acellular pertussis vaccination. Conclusion The mouse model for pertussis has been successfully developed in inhalation infection by means of the disease occurrence,isolation and cultivation cells from lungs and relevant infective curves. This mouse model could be applied in evaluation of pertussis vaccines,espacially,for efficacy analysis.
作者
高晶晶
李鑫
乔瑞洁
李燕婷
刘方蕾
罗树权
谢贵林
GAO Jing-jing;LI Xin;QIAO Rui-jie;LI Yan-ting;LIU Fang-lei;LUO Shu-quan;XIE Gui-lin(First Department of Research,Lanzhou Institute of Biological Products Co.,Ltd.,Center for Gansu Provincial Vaccine Engineering Research,Lanzhou 730046,Gansu Province,China)
出处
《微生物学免疫学进展》
2018年第5期28-35,共8页
Progress In Microbiology and Immunology
关键词
小鼠模型
呼吸道感染
百日咳疫苗
疫苗评价
Mouse model
Respiratory infection
Pertussis vaccine
Vaccine evaluation
