摘要
目的:观察毒热平注射液体外抗流感病毒的作用。方法:采用结晶紫染色法测定毒热平注射液不同加药方式对流感病毒亚甲型鼠肺适应株(FM1)的体外抑制作用及其作用24、36、48、72h的抗病毒效率。结果:毒热平注射液能明显阻止病毒吸附于细胞,并抑制细胞内病毒的复制增殖,在高浓度(≥0.18mg/mL)时有直接杀病毒作用。抗病毒效率在48h达高峰,且随时间延长在高浓度(≥0.27mg/mL)时基本不变,而在低浓度时有下降趋势。结论:毒热平注射液具有明显的体外抗流感病毒作用。
Objective ： To observe the antivirus effect of Dureping Injection in vitro experiments. Methods ： Observing the inhibitive effect of different drug-added ways of Dureping Injection against influenza virus FM1 strain in vitro and its antiviral effective rate at 24h,36h,48h and 72h, which were assayed by crystal violet staining method. Results ： Dureping Injection showed an obvious inhibitive effect against adsorption of the virus and inhibition on its prolongation inside MDCK. It was found that Dureping Injection had direct killing effect on the virus at high concentration （ ≥ 0.18 mg/mL ）.Its antiviral effect goes to the peak at 48h after infection, with the prolongation of acting time of drug the inhibitive effect of Dureping Injection kept no change at the high concentration （ ≥ 0.27 mg/mL ） and weakened at low concentration. Conclusion ： Dureping Injection has an obvious effect against influenza virus FM1 strain in vitro.
出处
《辽宁中医药大学学报》
CAS
2008年第3期6-8,共3页
Journal of Liaoning University of Traditional Chinese Medicine
基金
国家自然科学基金(30772872)
国家"863"计划创新药物与中药现代化重大专项(2005AA2Z3Foo)
关键词
毒热平
流感病毒
抗病毒
体外
Dureping
influenza virus
antiviral
in vitro
