摘要
目的探讨夏桑菊对甲型H1N1流感病毒的作用及其机制。方法 MTT法检测夏桑菊对MDCK细胞的半数有毒浓度(TC50)及对甲型H1N1流感病毒半数抑制浓度(IC_(50)),并计算选择指数(selection index,SI);Reed-Muench法检测甲型H1N1流感病毒对MDCK细胞的半数感染量(TCID50);空斑减少试验检测夏桑菊对甲型H1N1流感病毒的作用;荧光染色法观察夏桑菊对流感病毒NP蛋白的核定位作用;Western blot法检测夏桑菊作用流感病毒感染细胞后NF-κB通路相关蛋白的表达情况。结果 TC_(50)为4.98 mg/m L,IC_(50)为2.063 mg/m L,SI为2.414,TCID_(50)为0.044 668;甲型H1N1流感病毒经夏桑菊作用后,抑制了病毒株的空斑形成、病毒NP蛋白的核输出及NF-κB通路相关蛋白Iκκα、Iκκβ、NF-κBp50、NF-κBp65的磷酸化。结论夏桑菊对甲型H1N1流感病毒有明显的抑制作用,可能是通过抑制NF-κB通路相关蛋白磷酸化实现的。
Objective To investigate the effect of XiaSangju, a preparation of traditional Chinese drug, on influenza A （H1N1） virus as well as the relevant mechanism. Methods The median effective concentration （TCso） to MDCK cells and median inhibitory concentration （IC50） of influenza A （H1N1） virus of XiaSangju were determined by MTT assay, based on which the selection index （SI） was calculated. The median tissue culture infective dose （TCID50） of the virus to MDCK was determined by Reed-Muench method. The effect of XiaSangju on influenza A（HIN1 ） virus was investigated by plaque reduction neutralization test, while that on the location of NP by fluorescent staining. The expression of NF-KB pathway-associated proteins in the cells infected with influenza A（H1N1 ） virus was determined by Western blot. Results The TCso, IC5o, SI and TCIDso were 4. 98 mg / mL, 2. 063 mg / mL, 2. 414 and 0. 044 668 respectively. XiaSangju inhibited the plaque formation of influenza A （H1N1）, the export of NP from nuclei as well as the phosphorylation of NF- KB pathway-associated proteins IKKCt, IKKβ, NF-KBpso and NF-KBp65. Conclusion XiaSang, ju showed significantly inhibitory effect on influenza A （H1N1） virus by a possible mechanism of inhibiting the phosphorylation NF-KB pathway- associated proteins.
作者
于思明
李艳华
姜义
王磊
YU Si-ming;LI Yan-hua;JIANG Yi;WANG Lei(The First Hospital of Jilin University,Changchun 130021,Jilin Province,China)
出处
《中国生物制品学杂志》
CAS
CSCD
2018年第10期1099-1103,共5页
Chinese Journal of Biologicals
