摘要
为探讨银翘解毒软胶囊对甲型H1N1流感病毒PR8株感染小鼠肺炎模型的治疗作用机制,在滴鼻感染正常小鼠建立流感病毒肺炎模型的基础上,观察小鼠肺指数、病死率,并进一步进行分子机制研究。分别采用实时荧光定量PCR(real time RT-PCR)及Western blotting技术,在流感病毒感染后1、3、5、7天,检测小鼠肺组织中病毒载量的变化以及肺组织中M1蛋白相对含量的变化。结果显示,银翘解毒软胶囊(1和0.5 g.kg-1)在感染后第3、5、7天可明显降低正常小鼠感染后肺指数,并且两剂量组动物的死亡数显著降低,存活天数显著增加;银翘解毒软胶囊可以降低肺组织病毒载量,其中1 g.kg-1剂量组在各时间点均可降低其表达量(P<0.05,P<0.01),0.5 g.kg-1剂量组在第3、5、7天均可降低病毒载量(P<0.05,P<0.01);银翘解毒软胶囊可以降低肺组织M1蛋白相对表达量,其中1和0.5 g.kg-1剂量组在感染后第3、7天,与模型对照组相比明显低表达(P<0.05,P<0.01)。结果表明,银翘解毒软胶囊对甲型流感病毒有良好的治疗作用,并可能通过下调小鼠肺组织内病毒载量及M1蛋白相对表达量,来发挥其抗病毒作用。
This study is to investigate the treatment of YinQiaojiedu soft capsule for influenza virus A/PR8/34（H1N1） infection.The model of pneumonia was established by dropping influenza virus into the nose of normal mice,and the lung index and death rate were observed.Real time RT-PCR and Western blotting technique were used to detect the virus load and the relative expression of M1 protein in lungs of mice on the 1st,3rd,5th and 7th day after infection.The results showed that YinQiaojiedu soft capsule in 1 g·kg-1 and 0.5 g·kg-1 dose groups can decrease the lung index significantly on the 3rd,5th and 7th day after being infected（P〈 0.05,P 〈0.01）,and the number of death in the two groups of animals decreased significantly.YinQiaojiedu soft capsule in 1 g·kg-1 dose group can decreased virus load at each time point,and lower it in 0.5 g·kg-1 dose group at the 3rd,5th and 7th day（P 〈0.05,P 〈0.01）.YinQiaojiedu soft capsule can decrease the relative expression of M1 protein in lungs of mice,1 g·kg-1 and 0.5 g·kg-1 dose groups are significantly lower in expression of M1 protein compared with model group at the 3rd and 7th day（P〈 0.05,P 〈0.01）.It can be concluded that YinQiaojiedu soft capsule exerts antiviral effects against influenza virus by downregulating expression of virus load and M1 protein.
出处
《药学学报》
CAS
CSCD
北大核心
2011年第6期650-655,共6页
Acta Pharmaceutica Sinica
基金
国家"重大新药创制"科技重大专项(2009ZX09301-005-007)
中国中医科学院自主课题(Z02066
Z02093)
