摘要
目的:探讨银杏叶提取物(ginkgo biloba extract,GBE)对糖尿病肾病(diabetic nephropathy,DN)大鼠氧化应激及肿瘤坏死因子-α(tumor necrosis factor,TNF-α)表达的影响。方法:将72只大鼠随机分为对照组、DM组和DM+GBE组,每组各24只。采用一次性腹腔注射链脲佐菌素(60 mg·kg^(-1))的方法建立DM模型,造模成功后,DM+GBE组给予96mg·kg^(-1)·d^(-1)的GBE灌胃,对照组及DM组均以生理盐水以同等剂量灌胃。检测各组大鼠给药前后尿素氮(blood urea nitrogen,BUN)、肌酐(serum creatinine,Scr)、尿酸(uric acid,UA)水平及大鼠的肾组织匀浆丙二醛(malonaldehyde,MDA)、一氧化氮(nitric oxide,NO)、TNF-α、总氧化状态(total oxidation state,TOS)水平。并对部分肾脏进行HE染色,高倍显微镜下观察肾小管和肾小囊的变化。结果:给药第12周,与对照组比较,DM组大鼠体质量明显降低;与DM组比较,DM+GBE组体质量明显增加,差异均有统计学意义(P<0.05)。给药第12周,与对照组比较,DM组及DM+GBE组血糖水平升高;与DM组比较,DM+GBE组血糖水平降低,差异均有统计学意义(P<0.05)。与对照组比较,DM组UN、UA的水平增高;与DM组大鼠比较,DM+GBE组大鼠UN和UA浓度明显下降,差异均有统计学意义(P<0.05)。与对照组比较,DM组大鼠肾组织匀浆中MDA、TOS、NO、TNF-α水平升高;与DM组大鼠比较,DM+GBE组大鼠肾组织匀浆中MDA、TOS、NO、TNF-α水平明显降低,差异均有统计学意义(P<0.05)。结论:氧化应激和继发的炎症反应参与了糖尿病肾病的发病发展过程,银杏叶提取物能减轻氧化应激及继发的炎症反应从而对糖尿病肾病肾脏起到保护作用。
Objective： To observe the influence of ginkgo biloba extract（ GBE） on oxidative stress and TNF-alpha expression in STZ induced diabetic nephropathy rats. Methods： Seventy-two rats were randomly divided into the control group,DM group and DM ＋ GBE group,with 24 rats in each group. The DM model was established by intraperitoneal injection of streptozotocin（ 60 mg·kg-1）. After the model was successfully established,the DM ＋ GBE group was given 96 mg·kg-1·d-1 GBE for gavage,and the control group and the DM group were given the same dose of saline for gavage. The levels of blood urea nitrogen（ BUN）,serum creatinine（ Scr）,and uric acid（ UA） of the rats before and after administration and the renal homogenate malondialdehyde（ MDA）,nitric oxide（ NO）,TNF-α,（ total oxidation state（ TOS） levels were measured. Part of kidneys were stained with HE. And the changes of renal tubules and renal sacs were observed under high power microscope. Results： After the twelfth week of administration,compared with the control group,the body mass of the DM group decreased significantly. And the mass of the DM ＋ GBE group increased significantly compared with the DM group,and the difference was statistically significant（ P 〈0. 05）. After the 12 th week＇s administration,the blood glucose level of DM group and DM ＋ GBE group was higher than that of control group. Compared with the group DM,the blood glucose level of group DM ＋ GBE decreased,the difference was statistically significant（ P〈 0. 05）. Compared with the control group,the levels of UN and UA in the DM group increased,and the concentration of UN and UA in the DM ＋ GBE group was significantly lower than that in the DM group（ P〈 0. 05）. Compared with the control group,the levels of MDA,TOS,NO and TNF-α in the renal homogenate of the DM group increased. Compared with the DM group,the levels of MDA,TOS,NO and TNF-alpha in the renal homogenate of the DM ＋ GBE group were significantly lower than those in the DM �
作者
李瑞杰
张素英
李瑞静
赵晶晶
韩雷
刘光义
LI Rui-jie;ZttANG Su-ying;LI Rui-jing;ZHAO Jing-jing;HAN Lei;LIU Guang-yi(Xinle City TCM Hospital,Xinle,Hebei,China,05070)
出处
《河南中医》
2018年第7期1020-1023,共4页
Henan Traditional Chinese Medicine
关键词
糖尿病肾病
银杏叶提取物
氧化应激
尿素氮
肿瘤坏死因子-α
肌酐
尿酸
丙二醛
一氧化氮
总氧化状态
炎症反应
大鼠
diabetes mellitas(DM）
girikgo biloba extract(GBE）
Oxidative stress
blood urea nitrogen(BUN)
tumor necrosis factor -α(TNF-α）
serum creatinine(Scr）
uric acid(UA）
malondialdehyde(MDA）
nitric oxide(NO）
total oxidation state(TOS）
inflammatory reaction
rats
