摘要
为揭示环境激素邻苯二甲酸二丁酯(Dibutyl phthalate,DBP)与邻苯二甲酸二乙酯(Diethyl phthalate,DEP)对杜氏盐藻的抑制机理,研究了DBP与DEP对杜氏盐藻(Dunaliella tertiolecta)生长情况、β-胡萝卜素含量、超氧化物歧化酶(superoxide dismutase,SOD)活性、丙二醛(Malonaldehyde,MDA)含量和亚显微结构的影响。结果表明,DBP与DEP对藻细胞生长均有抑制作用,但二者作用机制不同:在DBP作用下藻体SOD活性持续增强,细胞膜结构损伤,MDA含量显著增加,表明DBP可能通过作用藻体的膜结构,引起细胞氧化损伤,最终导致细胞死亡。DEP使得藻体SOD活性先增强后减弱,β-胡萝卜素显著增加,亚显微结构中膜结构未发现明显损伤,但淀粉粒大量增加,线粒体畸形,藻体可能通过合成β-胡萝卜素和淀粉粒抵御氧化胁迫,但大量的物质合成可能导致细胞代谢紊乱,加速藻细胞衰亡。本研究为揭示邻苯二甲酸酯类环境激素的环境行为提供了科学依据。
The aim of this study was to investigate the mechanism of inhibitory action of dibutyl phthalate(DBP)and diethyl phthalate(DEP)on Dunaliella tertiolecta.The effects of DBP and DEP on the growth,β-carotene content,superoxide dismutase(SOD)activity,malonaldehyde(MDA)content and ultrastructure were investigated.The results showed that DBP and DEP both inhibited the growth of Dunaliella tertiolecta l,but the inhibitory mechanism varied.DBP caused disruption of algae ultrastructure,and increased the SOD activities and MDA contents,indicating that the algae growth inhibitions causing by the plasma membrane injury and oxidative damage under DBP exposure.Unlikely,DEP firstly increased the SOD activities and then decreased.Unlikely,DEP firstly increased the SOD activities before then inhibition.Accompanied by little change in MDA content,there was no significant oxidative damage on plasma membrane.Moreover,theβ-carotene and pyrenoid were increased rapidly to protect algae from DEP;but those activities may lead the alga to premature decay.This paper will give a theoretical support on the environmental behavior of PAEs on algae.
作者
唐燎
涂海峰
伍淼
顾书瑞
李锋民
TANG Liao;TU Hai-Feng;WU Miao;GU Shu-Rui;LI Feng-Min(Institute of Coastal Environmental Pollution,Ocean University of China,Qingdao 266100,China;Key Laboratory of Marine Environment and Ecology,Ministry of Education,Qingdao 266100,China;College of Environmental Science and Engineering,Ocean University of China,Qingdao 266100,China)
出处
《中国海洋大学学报（自然科学版）》
CAS
CSCD
北大核心
2018年第7期40-46,共7页
Periodical of Ocean University of China
基金
国家重点基础研究发展计划项目(2015CB453301)
国家基金委-山东省联合基金项目(U1606404)
国家自然科学基金项目(51378480)资助~~
关键词
DBP
DEP
杜氏盐藻
抑制机制
DBP
DEP
Dunaliella tertiolecta
inhibitory mechanism
