摘要
目的研究银杏叶提取物（extract of ginkgo biloba，EGB）抵抗肿瘤坏死因子-α（tumor necrosis factor-α，TNF-α）诱导的人肾小管上皮细胞凋亡及其作用机制。方法将人肾小管上皮细胞分成4组：对照组，TNF-α组，EGB＋TNF-α组，EGB组，各组细胞培养至80％～90％汇合时加入相应药物干预。采用MTT法检测TNF-α对细胞的毒性作用，通过细胞形态学检测（AO／EB染色）和流式细胞仪计数测定细胞凋亡发生情况，Western blot法测定细胞凋亡相关蛋白Bcl-2、Bax的表达。结果TNF-α对人肾小管上皮细胞的毒性作用呈剂量和时间依赖性；TNF-α（10μg／ml）可显著诱导人肾小管上皮细胞凋亡，而EGB（50mg／ml）可明显抑制它的作用，同时Western blot测定显示EGB可显著上调Bcl-2的表达而下调Bax的表达。结论EGB可以显著抑制TNF-α诱导的人肾小管上皮细胞凋亡，此作用可能与其上调抗凋亡蛋白Bcl-2的表达和下调促凋亡蛋白Bax的表达有关。
Objective To investigate the effects of Ginkgo biloba （EGB） extract against renal tubular epithelial cell apoptosis induced by tumor necrosis factor alpha （TNF-α）. Methods The human renal tubular epithelial cells were divided into 4 groups： control group, TNF-α group, EGB＋TNF-α group and EGB group. When the cells were grown to 80%-90% confluence, the cells were treated with the corresponding protocols. The cytotoxicity of TNF-α was evaluated by MTT assay. Apoptosis was assessed by AO/EB staining and flow cytometry. The expression of Bcl-2 and Bax was detected by Western blot. Results The cytotoxicity of TNF-α to human renal epithelial cells was dose- and time-dependent. The apoptosis of human renal epithelial cells was significantly induced by TNF-α （10 μg/ml）, which could be significantly attenuated by EGB （50 mg/ml）. Western blot revealed EGB could significantly up-regulate the expression of Bcl-2 and down-regulate the expression of Bax. Conclusion EGB significantly attenuated the apoptosis of human renal epithelial cells induced by TNF-α, which may be associated with the up-regulated expression of Bcl-2 and down-regulated expression of Bax.
出处
《华中科技大学学报（医学版）》
CAS
CSCD
北大核心
2008年第2期181-184,280,共5页
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
