摘要
目的:研究银杏叶提取物(ginkgo biloba extract,GBE)对糖尿病肾病(diabetic nephropathy,DN)大鼠氧化应激系统及炎症系统的影响。方法:将雌性Wistar大鼠72只随机分成3组,即对照组、DN组和DN+GBE组。采用一次性腹腔注射(60 mg/kg)链脲佐菌素的方法建立DN模型,并于实验第4周、8周和12周对动物进行处理,抽取腹主动脉血后将血样本冻存于-20℃的冰箱中,统一检测血葡萄糖、胆固醇、甘油三酯、胰岛素、SOD及肌酐。并对肾脏进行HE染色,镜下分析单个核细胞数和基底膜厚度,并检测3组大鼠体重变化及24 h尿蛋白量。将数据进行组间统计分析。结果:(1)在造模过程中DN组死亡大鼠1只,造模成功大鼠47只。(2)建模过程中,DN组体重增加最明显。第12周时,空腹血糖、甘油三酯、白细胞介素-6、超敏C-反应蛋白、血胰岛素、血肌酐和24 h尿蛋白在DN组最高,SOD在对照组最高,组间比较,差异均有统计学意义(P<0.05)。(3)在模型建立过程中,血白细胞介素-6、肌酐及空腹血糖呈现逐步上升的趋势,SOD呈现逐步下降的趋势,DM组及DM+GBE组各时间段间的差异有统计学意义(均P<0.05)。结论:GBE可能通过抗氧化应激及减弱炎症反应对DN的形成过程发挥作用。
Objective： To examine the effects of Ginkgo biloba extract（GBE） on oxidative stress and imflammation system in rats with diabetic nephropathy（DN）,and investigate its protective effects as well as its mechanism on DN. Methods： The seventy- two wistar rats were randomly divided into three groups, such as control group, DN group and DN treated with GBE group. Us- ing intraperitoneal injection of streptozotocin （60mg/kg） to induce diabete model. The kidneys were harvested for pathology obser- vation, 12 weeks later. The serum fasting blood glucose（FBG）,total cholesterol, triglyceride, interleukin-6,blood insulin, SOD, blood creatinine, 24 hours urine protein, the glomerular basement membrane thickening, and the numbers of total glomerular cells and monocytes were measured. Results： （1）In the modeling process, 1 rat died in DN group, 47 rats with model. （2）Blood glucose of the survival rats was more than 16.7 mmol/L. During the process of cultivating,one rat died. （2）In the process of mod- eling, the DN group weight increased obviously. At twelfth weeks, fasting blood glucose, triglyceride, interleukin-6, high sensitive C- reactive protein, serum insulin, serum creatinine and 24 h urine protein was highest in the DN group; SOD in the control group was the highest; comparison between groups showed statistically significant difference （P〈O.05）. （3） in the process of model- ing, the blood interleukin-6, creatinine and fasting blood glucose has gradually on the rise; SOD decreased; the difference was statistically significant in DM group and DM＋GBE group at different time segments （P〈0.05）. Conclusion： GBE may play a role by oxidative stress and reduced the formation process of inflammation in response to DN.
出处
《中医药导报》
2015年第3期31-34,共4页
Guiding Journal of Traditional Chinese Medicine and Pharmacy
关键词
糖尿病肾病
银杏叶提取物
氧化应激
炎症反应
模型
实验研究
diabetic nephropathy
Ginkgo biloba extract
oxidative stress
inflammation
model
experimental study
