摘要
将60只昆明小鼠随机分为正常对照组，LPS模型组，丹参多糖高、中、低（500，250，125mg／kg）保护组，每组12只。保护组用相应剂量的丹参多糖连续灌胃7d，1次／d。模型组和正常组给以等剂量的生理盐水。模型组和保护组在末次灌胃1h后，腹腔注射LPS（10mg／kg）建立肝损伤模型；正常对照组腹腔注射等体积的生理盐水。各组小鼠于注射LPS2h后，眼球取血分离血清，测定其血清谷丙转氨酶（AST）、谷草转氨酶（ALT）的活性和TNF-α、INF-γ、IL-6、IL-10含量；HE染色观察肝组织形态学变化；RT-PCR检测肝组织中TNF-α、INF-γ mRNA的表达。结果显示，与正常对照组相比，LPS模型组小鼠血清AST、ALT活性和TNF-α、INF-γ、IL-6、IL-10含量均显著增加（P〈0．05或P〈0．01）。丹参多糖各保护组（500，250，125mg／kg）均可显著降低AST、ALT的活性和TNF-α、INF-γ、IL-6、IL-10的含量，并可减少肝组织中TNF-α、INF-γ mRNA的表达。且丹参多糖对肝损伤的这种保护作用呈现一定的剂量依赖性。结果表明，丹参多糖对免疫性肝损伤具有显著的保护作用，且这种保护作用可能与丹参多糖降低炎症因子水平有关。
The aim of the present study was to study the protective effect of salvia miltiorrhiza polysaccharides on immunological liver injury induced by LPS,and to explore a possible mechanism. 60 Kunming mouse were randomly divided into 5groups：the normal group,the model group,the different does of salvia miltiorrhiza polysaccharides groups（500,250,125 mg/kg）,with 12 mice in each group. The treatment group were given salvia miltiorrhiza polysaccharides daily for 7 days. Immunological liver injury in mice was induced by LPS, at 2 h after administration of LPS, the mouses were killed. Liver pathological changes were observed by HE staining. The activity of ser- um ALT,AST and the content of TNF-α, INF-γ, IL-10 and IL-6 were determined by ELISA; Using RT-PCR to detect the mRNA expression of TNF-α and INF-γ in liver. Compared with the normal group, the activity of ALT, AST, the content of TNF-α, INF-γ, IL-10 and IL-6 in serum, and the mRNA expression of TNF-α and INF-y in liver were increased. Salvia miltiorrhiza polysac- charides（500,250,125 mg/kg） could obviously reduce the activities of ALT,AST, the content of TNF-α and IL-6 in serum and the mRNA expression of TNF rhiza polysaccharides has a protective effect on immunological tenuating inflammatory effect. a and INF-γ in liver. Salvia miltior liver injury,which is partially via attenuating inflammatory effect.
作者
韩超
杨金凯
王霄
陶金良
包永占
史万玉
HAN Chao;YANG Jin-kai;WANG Xiao;TAO Jin-liang;BAO yong-zhan;SHI Wan-yu(College of Traditional Chinese Veterinary Medicine,Agriculture University of Hebei,Baoding,Hebei 071000 China;Center for Traditional Chinese Veterinary Medicine Engineering Technology of Hebei,Baoding,Hebei 071000 China)
出处
《中国兽医学报》
CAS
CSCD
北大核心
2018年第7期1381-1385,共5页
Chinese Journal of Veterinary Science
基金
国家“十二五”科技支撑计划资助项目(2011BAD34B02)
关键词
免疫性肝损伤
丹参多糖
LPS
immunological liver injury
salvia miltiorrhiza polysaccharides
LPS
