摘要
目的评估独立通风笼盒(IVC)使用过程中,在设定参数下,同一笼架内各个笼盒换气次数及内外压差之间的差异。方法屏障环境内,以单面IVC为检测对象,设备参数设置:换气次数50次/h、40次/h,压差+20 Pa、+10 Pa,检测笼盒送风口的风速及笼盒内外压差。结果设定换气次数与实测笼盒内换气次数存在差异,实测值在设定值的±30次/h范围内波动,并且同一笼架上笼盒与笼盒之间的换气次数实测值差异较大,在30次/h^60次/h;不同位置笼盒间内外压差的差异较小,实测值均在设定值的±10 Pa范围内波动。结论为保证笼盒内微环境的一致性,减少环境差异对实验结果的影响,需进一步研究如何将同一架IVC笼盒之间的换气次数和内外压差波动控制在较低范围。
Objective To assess the variation of air changes and pressure parameters in the same equipment of individually ventilated cage（IVC） during the daily operation. Methods Taking the onsite single-side IVC as the test object which was placed in the barrier environment, and the parameters were set like this： ventilation rate 50 times/h, 40 times/h; the pressure： ＋ 20 Pa, ＋ 10 Pa. The sample points were set at the air inlet, which was used to test the air speed, meanwhile testing the pressure between the cage inside and outside. Result During the IVC equipment daily operation, the variation of air changes among the cages were huge, the number ranged from 30 times/h to 60 times/h, but the parameter of pressure between inside and outside has little difference among the cage, and within the set value ± 5 Pa range fluctuations. Conclusion The structure and ventilation system of IVC equipment had big impact on the air changes, the temperature, humidity, airflow velocity and ammonia concentration in IVC cage were closely related to the ventilation rate. In order to reduce the environmental differences on the experimental results, controlling the ventilation rate and pressure difference between internal and external of cages are necessary.
作者
张建平
傅江南
ZHANG Jian-ping;FU Jiang-nan(Institution of Laboratory Animals, Jinan University, Guangzhou 510632, China)
出处
《实验动物与比较医学》
CAS
2018年第3期231-235,共5页
Laboratory Animal and Comparative Medicine
