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山核桃(Carya cathayensis)种子生长发育过程转录组的初步分析 被引量:5

Preliminary Analysis of Transcriptome of Hickory(Carya cathayensis) Seed in Growth and Development Process
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摘要 山核桃(Carya cathayensis)是浙江省重要的经济树种,其种子脂类含量极高,达69.80%~74.01%。本研究采用Illumina Hi Seq TM 2000高通量测序技术对高油种子生长发育过程(6个时间点)采集的种胚及胚乳进行转录组分析,经组装拼接共获得Unigene 110 959条,占总转录本数的53.93%,并有39 560个Unigene至少在7个数据库中的一个数据库中获得注释。这些注释的基因GO分类共涉及47个生物学功能,KOG分类成26组,KEGG代谢通路分成5个分支。基因差异表达分析表明,胚乳在种子生长发育被吸收的过程中基因表达量不低,上下调基因数均是先增加后减少,而同一时间点上下调胚与胚乳差异表达基因(DEG)数均逐渐增加。DEG聚类分界点在7~8月之间。初步发现山核桃种子生长发育过程涉及脂类合成的途径21个,相关基因571个。这些结果为山核桃日后成油机理的研究打下了基础。 Hickory(Carya cathayensis), an important economic tree species in Zhejiang province, has a high oil content of 69.80%~74.01% in its seeds. RNA-seq was performed with seed embryo and endosperm collected from the developing high-oil seeds(in 6 times) by using the high throughput sequencing technology of Illumina Hi SeqTM2000. 110 959 Unigenes were assembled, which accounted for 53.93% of total transcripts, and 39 560 Unigenes were annotated in at least one of total 7 databases. 47 biological functions were found according to GO-based annotation. 26 groups were classified according to KOG-based annotation, and the KEGG metabolic pathway was divided into 5 branches. Analysis of genes differential expression showed that gene expression in endosperm was not low in the process of seed growth and development, and the number of both up and down-regulated genes was increased first, and followed by a decrease, but the number of both up and down-regulated DEGs between embryo and endosperm sampled on the same time increased gradually. The clustering demarcation point of DEGs was between July and August. 21 pathways and 571 genes related with lipid synthesis were found during the growth and development of seeds. All these results would provide a basis for further study on oil mechanism in hickory.
作者 董昂 赵国淼 胡欢欢 陈文充 叶生月 曾燕如 Dong Ang;Zhao Guomiao;Hu Huanhuan;Chen Wenchong;Ye Shengyue;Zeng Yanru(The State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Lin'an, 311300;Tonglu Ago-Industrialization Office, Tonglu, 311500)
出处 《分子植物育种》 CAS CSCD 北大核心 2018年第12期3844-3854,共11页 Molecular Plant Breeding
基金 浙江省自然科学基金重点项目(LZ13C160002) 国家自然科学基金项目(31370678)共同资助
关键词 山核桃 种子 转录组 RNA-SEQ 基因注释 Hickory Seed Transcriptome RNA-seq Gene annotation
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