摘要
为了鉴定甘蓝型油菜基因组中新的未知转录本,以甘蓝型油菜叶片为材料提取总RNA,构建c DNA文库并使用Illumina双端测序Hiseq 2500平台进行测序。用生物信息学的方法分析高质量的测序reads来鉴定新的未知转录本。结果表明,在甘蓝型油菜基因组已知基因的间隔区共鉴定到7 720个候选新转录本,通过RT-PCR和克隆测序验证了15个新鉴定的转录本。
In order to identify novel unknown transcripts in Brassica napus genome,total RNAs were extracted from leaves of Brassica napus,c DNA libraries were constructed,and sequenced using Illumina dual terminal sequencing Hiseq 2500 platform. Novel unknown transcripts were identified by bioinformatics analysis of high quality sequenced reads,and the results showed that 7 720 candidate novel transcripts were identified in the spacer region of known genes in Brassica napus genome. Fifteen newly identified transcripts were confirmed by RT-PCR and clone sequencing.
作者
徐文
安素妨
王艳
贾琳琳
鲁丹丹
张莹莹
刘建丰
李保全
XU Wen1,AN Sufang1,WANG Yan1,JIA Linlin1,LU Dandan1,ZHANG Yingying1,LIU Jianfeng2,LI Baoquan1(1.Crop Designing Centre,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China;2.Agricultural Economy ＆ Information Research Institute,Henan Academy of Agricultural Sciences,Zhengzhou 450002,Chin)
出处
《河南农业科学》
CSCD
北大核心
2018年第4期38-42,共5页
Journal of Henan Agricultural Sciences
基金
河南省基础与前沿技术研究计划项目(162300410153)
