摘要
采用腹腔注射环磷酰胺(Cyclophosvnamide,CTX)建立免疫低下小鼠模型,研究不同剂量牡蛎寡肽对小鼠免疫器官脏器指数、脾淋巴细胞转化增殖情况、自然杀伤细胞(Natural Killer cell,NK)细胞活性、小鼠体液免疫、巨噬细胞吞噬能力、血清TNF-α、IL-6和溶血素水平的影响.结果显示:与免疫抑制模型组小鼠相比,牡蛎寡肽能够显著提高脾淋巴细胞增殖能力、NK细胞活性、廓清指数K、吞噬指数a、吞噬中性红能力、TNF-α、IL-6、溶血素水平和脾淋巴细胞CD3+4T淋巴细胞亚群及CD3+8T淋巴细胞亚群分布(P<0.05),而对小鼠的肝、脾脏指数影响不显著,表明牡蛎寡肽能够提高由CTX引起的免疫低下模型小鼠的细胞免疫、体液免疫及非特异性免疫功能,对小鼠的免疫功能具有正面调控的作用.
The effect of oyster oligopeptide on regulation of immune function in cyclophosphamide（ CTX）-induced immunosuppressive mice was evaluated. The indexes of immune organs such as liver and spleen,the transformation and propagation ratio of T lymphocyte,the activity of NK cell,the humoral immunity,macrophage phagocytosis,the level of cell cytokines and hemolysin were assessed. The experiment results revealed that compared with the model immunosuppressed group,oyster oligopeptide could significantly enhance the proliferation of spleen lymphocyte,NK cell activity,expurgation index k,devouring index a,ability of devouring neutral red,the level of TNF-α、IL-6 and hemolysin,and regulate the distribution of CD3＋4 T and CD3＋8 T spleen lymphocyte subsets. However,the influence of oyster oligopeptide on the immune organs was not significant. It was concluded that oyster oligopeptide can improve the cell immunity,humoral immunity and non-specific immune function of immunosuppressive mice induced by CTX,and it can regulate the immune function positively in mice.
作者
刘淑集
许旻
苏永昌
王茵
潘南
乔琨
吴靖娜
苏捷
张伯超
蔡水淋
刘智禹
LIU Shuji;XU Min;SU Yongchang;WANG Yin;PAN Nan;QIAO Kun;WU Jingna;SU Jie;ZHANG Boehao;CAI Shuilin;LIU Zhiyu(Fisheries Research Institute of Fujian//Key Laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian Province// Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen 361013, China;College of Food Science//Fujian Agriculture and Forestry University, Fuzhou 350002, China)
出处
《华南师范大学学报（自然科学版）》
CAS
北大核心
2018年第2期70-76,共7页
Journal of South China Normal University(Natural Science Edition)
基金
福建省科技重大专项(2014NZ0001-1)
福建省海洋高新产业发展专项(闽海洋高新[2014]20号
闽海洋高新[2016]07号)
福建省海洋发展补助资金(留省部分)项目(MHJFL2017-1)
关键词
牡蛎寡肽
免疫调节
环磷酰胺
免疫抑制
oyster oligopeptide
immunoregulation
cyclophosphamide
immunosuppression
