摘要
目的探讨微小RNA（miR）-21在肝癌组织中的表达及其临床意义。方法选择81例肝癌组织及癌旁组织和20例正常肝组织标本。实时定量反转录聚合酶链反应（RT．qPCR）法检测miR-21表达，分析miR-21表达与患者临床病理特征及预后的关系。结果与正常肝组织比较，miR-21肝癌组织中表达升高了（6．34±0．23）倍（P=0．003），在癌旁组织中升高（3．444-0．12）倍（P=0．020）。miR-21表达与肿瘤直径（X^2=11．034，P=0，001）、转移（X^2=12．081，P=0．001）、血管侵犯（X^2=13．372，P=0．000）及美国癌症联合委员会（AJCC）分期（X^2=12．175，P=0．000）明显相关，而与性别（X^2=0．290，P=0．590）、年龄（X^2=0．036，P=0．850）、肿瘤位置（X^2=0．593，P=0．441）、肿瘤分化（X^2=2．729，P=0．099）、肿瘤数量（X^2=1．597，P=0．206）及甲胎蛋白（AFP,X^2=0．279，P=0．597）无明显相关。肝癌组织miR-21高表达的患者5年生存率明显低于低表达患者（27．2％比10．7％，P=0．003），miR-21高表达是影响肝癌患者总体生存的独立风险因素（P=0．001）。结论miR-21在肝癌组织中表达明显上调，并且与肿瘤转移明显相关，参与了肝癌的发展过程。
Objective To detect the expression of microRNA （miRNA, miR） -21 in hepatocel- lular carcinoma （HCC） tissue samples and to explore its association with clinicopathologic features. Methods The real - time quantitative reverse transcriptase - polymerase chain reaction （ RT - qPCR ） analysis was used to detect the expression of miR - 21 in tissue specimens from HCC and the adjacent non- cancerous hepatic tissues collected from 81 patients who underwent surgical treatment for primat3, HCC and in normal liver tissues collected from 20 patients. Statistically, the association between the ex- pression of miR - 21 and clinicpathologic characteristics and prognosis of patients was analyzed. Results The expression of miR - 21 was up - regulated in HCC [ （6. 34 ± 0. 23 ） fold, P = 0. 003 ] and the adjacent non -cancerous hepatic tissues [ （3.44 ±0. 12） fold, P =0. 0201 as compared with that in the normal liv- er tissues. The results showed that high expression of miR -21 was strongly correlated with the characteris- tics of tumor size （X2 = 11. 034, P = 0. 001 ）, metastasis （X^2= 12. 081, P = 0. 001 ）, venous invasion （X^2 = 13. 372, P = 0. 000） and American Joint Committee on cancer （AJCC） TNM stage （X^2 = 12. 175, P = 0. 000）. There were no significant associations between miR -21 expression and gender （Xz = 0. 290, P =0. 590）, age （X^2 =0. 036, P =0. 850）, tumor location（X^2 =0. 593, P =0. 441）, tumor grade （X^2 = 2. 729, P = 0. 099）, tumor number （X^2 = 1. 597, P = 0. 206） and alpha fetal protein （ AFP, X^2 = 0. 279, P = 0. 597 ）. Patients with high miR -21 expression had significantly poorer overall survival （27.2% vs. 10. 7%, P =0. 003）. High miR -21 expression was independent prognostic factors for overall survival （P = 0. 001 ）. Conclusion The expression of miR - 21 is highly up - regulated in HCC tissues, which is correlated significantly with the process of metastasis and unfavorable prognosis.
作者
李庆军
周进学
杨楠木
展翔宇
王征征
陈勋
朱瑞利
韩风
黄涛
Li Qingjun;Zhou Jinxue;Yang Nanmu;Zhan Xiangyu;Wang Zhengzheng;Chen Xun;Zhu Ruili;Han Feng;Huang Tao(Department of Hepatobiliary and Pancreatic Surgery, the Affiliated Tumor Hospital, Zhengzhou University, Zhengzhou 450008, Chin)
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2018年第6期1140-1142,共3页
Chinese Journal of Experimental Surgery
基金
国家自然科学基金（U1304818）
关键词
癌
肝细胞
微小RNA-21
临床意义
Carcinoma
hepatocellular
MicroRNA -21
Clinical significance
