摘要
目的观察miR-21与程序性死亡因子4(PDCD4)在结肠癌组织中的表达情况并探讨二者关系。方法采用免疫组织化学检测78例结肠癌患者及癌旁组织中PDCD4蛋白表达情况;实时荧光定量PCR(qRT-PCR)检测组织miR-21和PDCD4 mRNA的表达,并分析二者之间相关性;采用苏木素-伊红(HE)染色判定结肠病变程度及性质。收集患者临床数据信息并分析其与miR-21和PDCD4 mRNA表达之间的关系。结果免疫组织化学结果显示,70例(89.74%)结肠癌组织中PDCD4蛋白低表达或无表达。qRT-PCR显示,72例(92.31%)结肠癌组织中miR-21表达水平较癌旁组织高,68例(87.18%)PDCD4 mRNA表达水平较癌旁组织低。miR-21和PDCD4 mRNA表达在淋巴结是否转移中比较,差异有统计学意义(P<0.05),但二者表达无相关性(P>0.05)。结论miR-21高表达或PDCD4低表达可能与结肠癌发生有关。
Objective To observe the expression of miR-21 and programmed death factor 4(PDCD4)in patients with colon cancer and explore their relationship.Methods Immunohistochemistry was used to detect the expression of PDCD4 in cancer tissues and adjacent normal tissues of 78 patients with colon cancer.The real-time quantitative PCR(qRT-PCR)was used to detect the expression of miR-21 and PDCD4 mRNA in tissues,and the correlation between them was analyzed.Hematoxylin-eosin(HE)staining was used to determine the degree and nature of colon lesions.The clinical data of patients were collected and their relationship with the expression of miR-21 and PDCD4 mRNA was analyzed.Results Immunohistochemical results showed that 70 cases(89.74%)of colon cancer tissues had low or no expression of PDCD4 protein.qRT-PCR showed that the expression level of miR-21 in colon cancer tissues of 72 cases(92.31%)was higher than that in adjacent tissues,and the expression level of PDCD4 mRNA in 68 cases(87.18%)was lower than that in adjacent tissues.The expressions of miR-21 and PDCD4 mRNA were compared in lymph node metastasis,the difference was statistically significant(P<0.05),but there was no correlation between them(P>0.05).Conclusion High expression of miR-21 or low expression of PDCD4 may be related to the occurrence of colon cancer.
作者
郭美
王兵
周恒花
王萍
GUO Mei;WANG Bing;ZHOU Henghua;WANG Ping(Department of Gastroenterology,Shanghai Ninth People′s Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200011,China;Department of General Surgery,Shanghai Ninth People′s Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200011,China;Department of Pathology,Shanghai Ninth People′s Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200011,China)
出处
《重庆医学》
CAS
2022年第6期1011-1014,共4页
Chongqing medicine
