摘要
目的建立快速、准确测定电子烟油中尼古丁的超高效液相色谱-电喷雾电离质谱(ultra high performance liquid chromatography-electrospray ionization/mass spectrometry,UPLC-ESI/MS)分析方法。方法使用UPLC BEH HILIC色谱柱作为固定相,以10 mmol/L乙酸铵-甲醇为流动相,等度洗脱,在电喷雾(electronic spray ion,ESI)正离子模式下,采用选择离子监测方法进行测定,同位素D4-尼古丁稀释法定量。结果烟油中尼古丁的检出限为0.5μg/L。检测范围为2~100μg/L,样品在10、50和100 mg/kg时的加标回收率分别为84.7%、96.1%和92.7%,相对标准偏差均小于5%。结论该方法快捷、准确、灵敏度高,可用于电子烟油中尼古丁的快速准确测定。
Objective To establish a rapid and accurate method of determination of nicotine in the electronic cigarette by ultra performance liquid chromatography-electrospray ionization mass spectrometry（UPLC-ESI/MS）. Methods UPLC BEH HILIC column was used as the stationary phase, and the liquid phase was 10 mmol/L ammonium acetate for isocratic elution. The nicotine was detected by ESI＋ under selected ion monitoring mode, and was quantitated using isotope-labeled nicotine（D4-nicotine） internal standard. Results The limit of detection of nicotine in electronic cigarette was 0.5 g/L. The detection range was 2-100 μg/L. The recovery rates of the samples at 10, 50 and 100 mg/kg were 84.7%, 96.1% and 92.7%, respectively. The relative standard deviations were less than 5%. Conclusion This method is rapid, accurate, and highly sensitive, which is suitable for the determination of nicotine in electronic cigarettes.
作者
蒋萍萍
冯峰
娄婷婷
张峰
JIANG Ping-Ping;FENG Feng;LOU Ting-Ting;ZHANG Feng(Institute of Food Safety, Chinese Academy of Inspection and Quarantine, Beijing 100123, China;Animal Plant and Food Inspection Center, Tianjin Entry-exit Inspection and Quarantine Bureau, Tianjin 300461, China)
出处
《食品安全质量检测学报》
CAS
2018年第10期2326-2330,共5页
Journal of Food Safety and Quality
基金
2018质量监督项目~~
关键词
超高效液相色谱-串联质谱法
同位素稀释法
电子烟
尼古丁
ultra performance liquid chromatography-tandem mass spectrometry
isotope dilution
electronic cigarette
nicotine
