摘要
A sensitive, simple and low-cost method based on capillary electrophoresis（CE） with electrochemical（EC） detection at a carbon fiber microdisk electrode（CFE） was developed for the determination of nicotine. Effects of de- tection potential, concentration and pH value of the phosphate buffer, and injection time as well as separation voltage were investigated. Under the optimized conditions： a detection potential of 1.20 V, 40 rnmol/L phosphate buffer（pH 2.0）, a sample injection time of 10 s at 10 kV and a separation voltage of 16 kV, the linear range obtained was from 5.0×10^-7 mol/L to 1.0×10^-4 mol/L with a correlation coefficient of 0.9989 and the limit of detection（LOD, S/N=3） obtained was 5.0×10^-8 mol/L. The method was also used to determine the nicotine in cigarettes. Nicotine amount ranged from 0.211 mg/g to 0.583 mg/g in the pipe tobacco of seven brands of cigarette and the amount in one ciga- rette varied from 0.136 mg/cigarette to 0.428 mg/cigarette.
A sensitive, simple and low-cost method based on capillary electrophoresis（CE） with electrochemical（EC） detection at a carbon fiber microdisk electrode（CFE） was developed for the determination of nicotine. Effects of de- tection potential, concentration and pH value of the phosphate buffer, and injection time as well as separation voltage were investigated. Under the optimized conditions： a detection potential of 1.20 V, 40 rnmol/L phosphate buffer（pH 2.0）, a sample injection time of 10 s at 10 kV and a separation voltage of 16 kV, the linear range obtained was from 5.0×10^-7 mol/L to 1.0×10^-4 mol/L with a correlation coefficient of 0.9989 and the limit of detection（LOD, S/N=3） obtained was 5.0×10^-8 mol/L. The method was also used to determine the nicotine in cigarettes. Nicotine amount ranged from 0.211 mg/g to 0.583 mg/g in the pipe tobacco of seven brands of cigarette and the amount in one ciga- rette varied from 0.136 mg/cigarette to 0.428 mg/cigarette.
基金
Supported by the National Natural Science Foundation of China(No.20875085)
