血细胞分析仪XE-5000与流式细胞仪FACSCalibur在测定造血干细胞中的作用被引量：2

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摘要目的探讨Sysmex XE-5000全自动血液分析仪和FACSCalibur流式细胞仪在测定造血干细胞中的相关性。方法分别应用XE-5000全自动血细胞分析仪上的HPC分析模式和流式细胞术对全血、外周血造血干细胞(PBSC)及白血病患者骨髓中的HPC及CD34+含量进行检测。结果测定的样本为自体动员的全血时,XE-5000血细胞分析仪测定的HPC绝对数与流式细胞仪测定的CD34+细胞绝对数呈正相关(r=0.52)。测定的样本为PBSC时,对于稀释5倍的PBSC样本其相关系数r=0.057。标本批内变异系数(CV)为3.9%。同一患者自体干细胞动员后的第4天WBC、CD34+和HPC同时明显上升,动员第5天同时达到高峰。结论在自体外周血干细胞移植时,流式细胞仪能准确测定造血干细胞的含量,Sysmex XE-5000虽然具有快捷、简捷的特点,但仍不能替代流式细胞术测定造血干细胞。

作者罗俭权李海洋罗耀凌

机构地区四会市人民医院检验科四会市人民医院血液科中山大学肿瘤防治中心检验科

出处《广东医学》 CAS 2018年第10期1518-1521,共4页 Guangdong Medical Journal

关键词 SYSMEX XE-5000 流式细胞术 HPC CD34+

分类号 R446.1 [医药卫生—诊断学]

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