摘要
目的探讨高效液相色谱(HPLC)波长切换联合梯度洗脱法同时测定理气散结颗粒中芍药苷、柴胡皂苷a、柴胡皂苷d、延胡索乙素、去氢紫堇碱和紫堇碱6种成分的含量可行性。方法采用Agilent TC-C18色谱柱;以乙腈(A)-0.1%冰醋酸溶液(B)为流动相,梯度洗脱;检测波长分别为230 nm(芍药苷)、210 nm(柴胡皂苷a和柴胡皂苷d)、280 nm(延胡索乙素、去氢紫堇碱和紫堇碱);流速:1.0 m L·min-1;柱温:35℃。结果芍药苷、柴胡皂苷a、柴胡皂苷d、延胡索乙素、去氢紫堇碱和紫堇碱分别在14.39~359.75 mg·L-1、1.96~49.00 mg·L-1、1.44~36.00 mg·L-1、1.36~34.00 mg·L-1、1.58~39.50 mg·L-1、2.12~53.00 mg·L-1范围内线性关系良好,r分别为0.999 8、0.999 5、0.999 9、0.999 4、0.999 7、0.999 9(n=6),其平均回收率分别为99.87%、97.75%、98.09%、97.46%、98.29%和96.97%,RSD分别为0.84%、1.74%、0.71%、1.39%、1.07%和1.11%。结论该方法简便、准确、重复性好,可作为理气散结颗粒全面质量控制方法。
Objective To establish a method for the simultaneous determination of paeoniflorin ,saikosaponina,saikosaponind,tetrahydropalmatine,dehydrocorydaline and corydaline in Liqi Sanjie Granules by HPLC wavelength switching combined with gradient elution method.Methods The Agilent TC-C18 column was used,the mobile phase was acetonitrile （A） -0.1% glacial acetic acid solution （B） with gradient elution,and the detection wavelengths were 230 nm for paeoniflorin,210 nm for saikosaponina and saikosaponind ,and 280 nm for tetrahydropalmatine,dehydrocorydaline and corydaline ,respectively.The flow rate was 1.0 mL· min-1 ,and column temperature was set at 35 ℃.Results The linear relationship of paeoniflorin,saikosaponina,saikosaponind,tetrahydropalmatine,dehydrocorydaline and corydaline was good in the ranges of 14.39 -359.75 mg· L-1 （r =0.9998）,1.96 -49.00 mg· L-1 （r =0.9995）,1.44 -36.00 mg· L-1 （r =0.9999）,1.36 -34.00 mg· L-1 （r =0.9994）,1.58 -39.50 mg· L-1 （r =0.9997）,2.12 -53.00 mg· L-1 （r =0.9999）,respectively.The average recoveries were 99.87%,97.75%,98.09%,97.46%,98.29% and 96.97%,and RSDs were 0.84%,1.74%,0.71%,1.39%,1.07% and 1.11%.Conclusions The method is simple,accurate andwell reproducible,which can be used as quality control method for Liqi Sanjie Granules .
作者
王育
王毅
严亨波
魏谭军
李霞
WANG Yu;WANG Yi;YAN Hengbo;WEI Tanjun;LI Xia(Department of Traditional Chinese Medicine Analysis, Dazhou Institution of Food and Drug Control, Dazhou, Sichuan 635000, China;Department of Pharmaceutical Preparation, Dazhou Hospital of Integrated TCM ＆ WM, Dazhou,Sichuan 635000, China)
出处
《安徽医药》
CAS
2018年第6期1027-1030,共4页
Anhui Medical and Pharmaceutical Journal
基金
四川省科技支撑计划项目(2014SZ0189)
关键词
色谱法
高压液相
理气散结颗粒
芍药苷
柴胡皂苷A
柴胡皂苷D
延胡索乙素
去氢紫堇碱
紫堇碱
波长切换法
梯度洗脱法
Chromatography
high pressure liquid
Liqi sanjie granules
Paeoniflorin
Saikosaponina
Saikosaponind
Tetrahydropalma-tine
Dehydrocorydaline
Corydaline
Wavelength switching method
Gradient elution method
