摘要
采用响应面法优化盐碱地栽培忍冬(Lonicera japonica Thunb.)器官综合物中咖啡酸、绿原酸、木犀草苷、多糖4种成分同时超声提取的最佳条件。以咖啡酸、绿原酸、木犀草苷、多糖4种成分总含量为响应值,通过Box-Behnken响应面法对提取时间、温度、料液比、乙醇体积分数这4个主要因素进行考察。结果表明,最优提取条件为提取时间1 h,温度75℃,料液比1∶40(g∶m L),乙醇体积分数为45%。应用超声提取技术能够在盐碱地栽培的忍冬器官综合物中同时获得一定含量的绿原酸、多糖、咖啡酸、木犀草苷等产物,具有提取效率高、节约资源等优势。
An ultrasonic-assisted extraction method was established to simultaneously produce the chlorogenic acid,cafferic acid,luteoloside and polysaccharose from Lonicera japonica Thunb. in saline-alkali land. Using the total concentration chlorogenic acid,cafferic acid,luteoloside and polysaccharose of as response value,Box-Behnken response surface design was adopted to investigate the four main factors of extraction time,temperature,solid/liquid ratio and ethanol concentration. The results showed that the optimization conditions for extraction were extraction time 1 h,temperature 75 ℃,and solid/liquid ratio 1∶40（g∶m L） and ethanol concentration 45%. The ultrasonic-assisted extraction of the four components has the advantage of accelerating the extraction process and better products with lower cost.
作者
胡玉涛
李天雪
许天罡
HU Yu-tao;LI Tian-xue;XU Tian-gang(The Center for Drug Research ＆ Development,Lianyungang College of Traditional Chinese Medicine of Jiangsu 222007, Jiangsu, China;Zibo Wellcell Biotechnology Co., Ltd., Zibo 256302,Shandong, China)
出处
《湖北农业科学》
2018年第9期88-91,共4页
Hubei Agricultural Sciences
基金
连云港市科技计划农业攻关项目(CN1406)
连云港市"521工程"科研项目资助计划(2016)
江苏省中医药局科技项目(YB2017068)
