摘要
目的优选金银花中总黄酮和多糖的微乳提取工艺。方法运用配伍法初步筛选微乳处方,比较浸渍法、超声法、回流法分别应用于微乳处方的提取率,并与70%乙醇提取总黄酮、水提取多糖的得率比较。以料液比、提取时间、微乳稀释倍数为考察因素,总黄酮和多糖含量为指标,采用正交试验法优选金银花的微乳提取工艺并进行验证。结果提取金银花中总黄酮和多糖的最佳工艺为:以油酸乙酯-吐温80-无水乙醇(质量比为4∶27∶9)为溶剂,料液比1∶50、提取时间180 min、微乳加水稀释25倍回流提取,与应用70%乙醇和水作为溶剂相比,提取率分别增加了16.14%和16.30%。结论微乳作为提取溶剂可同时提取金银花中的总黄酮及多糖,工艺流程简单,提取效率高。
Objective To optimize the microemulsion extraction technology of total flavonoids and polysaccharides from Lonicera japonica Thunb.Methods The microemulsion formulas are screened by compatibility methods and the efficiency of microemulsion extracting is compared with immersion,ultrasonic and reflux method.The extraction rate of total flavonoids with 70% ethanol and polysaccharides with water are further compared with microemulsion formulas.The content of total flavonoids and polysaccharides are used as indexes, the microemulsion extraction technology is optimized by orthogonal test with the factors of extraction time,the ratio of medicinal materials to microemulsion solution and dilution ratio.Results The optimal microemulsion extraction technology condition is as follows:ethyl oleate-tween80-absolute ethanol (mass ratio of 4:27:9)is used as solvent,the material-liquid ratio of 1:20,extraction time of 180 min,dilution ratio with water of 25. Compared with 70% ethanol and water as solvents,the extraction rate of total flavonoids and polysaccharides increased by 16.14% and 16.30%,respectively.Conclusion Total flavonoids and polysaccharides from Lonicera japonica Thunb.can be extracted simultaneously with microemulsion as extraction solvent,the extraction process is simple and the extraction efficiency is high.
作者
赵惠茹
崔阳
靖会
杨阳
苏慧珊
张琳
ZHAO Hui-ru;CUI Yang;JING Hui;YANG Yang;SU Hui-shan;ZHANG Lin(College of Pharmacy,Xi'an Medical University,Xi'an 710021,China)
出处
《现代中药研究与实践》
CAS
2018年第6期58-61,共4页
Research and Practice on Chinese Medicines
基金
西安医学院药学省级重点学科建设项目(2016YXXK04)
西安医学院大学生创新训练计划项目(2018DC-18)
西安医学院配套基金项目(2016PT13
2016PT15)
国家级大学生创新训练计划项目(201825018)
关键词
正交试验
金银花
总黄酮
多糖
微乳
Orthogonal test
Lonicerajaponica Thunb.
total flavonoids
polysaccharides
microemulsion
