摘要
[目的]探知罗布人mtDNA遗传结构与遗传多样性,构建人群mtDNA遗传数据库。[方法]运用二代测序技术对164位罗布人mtDNA全序列进行检测、分型。[结果]共检出315处突变位点包含298处多态性位点,其中A263G、A750G、A4769G、A8860G、A15326G位点突变率为100%,核苷酸多样性为0.0084,平均核苷酸配对差异数目为9.4695;划分出27个单倍型,高频单倍型有:B5b2c/31.71%、H6a1b2/18.90%、D4c2a/13.41%、H9a/9.76%,单倍型多样性为0.8360,个体识别力为0.8309;主成分和邻接系统树分析显示:罗布人与维吾尔族、蒙古族、中亚人群遗传关系近,特别与维吾尔族亲缘关系最近。[结论]现代罗布人mtDNA遗传结构具有亚欧混合现象,遗传多样性丰富;单倍型主要集中在B(39.03%)、H(29.88%)、D(15.85%);mtDNA遗传结构数据可为人群在遗传进化、族源鉴定、个体识别等方面提供依据。
[Objective]To explore the genetic structure and genetic diversity of Lopnur in Xinjiang,and to construct genetic database of mtDNA.[Methods] The 164 mtDNAs of Lopnur have been sequenced and typed by two generation sequencing technique.[Results] Total of 315 base mutations and 298 polymorphic locis were detected.Among them,the mutations of A263 G,A750 G,A8860 G,A15326 G were 100%,and nucleotide diversity was 0.0084,the average number of nucleotide pairs was 9.4695.27 haplotypes were detected fromLopnur with B5b2c（31.71%）,H6a1b2（18.90%）,D4c2a（13.41%）,H9 a（9.76%） high-frequently appeared.The haplotype diversity was 0.8360,and the individual identification power was 0.8309.Principal component analysis and Test Neighbor-Joning Tree analysis revealed that：Lopnur have a close relationship with Uygur,Mongolian and Central Asian populations,especially with the Uygur.[Conclusion]The mtDNA genetic structure of modern Lopnur peoples have the Eurasian mixed phenomenon,and the mtDNA Genetic diversity were highly polymorphic.Haplotypes were mainly concentrated in B（39.03%）,H（29.88%）,and D（15.85%）.The Mt DNA genetic structure datas could provide a basis for population in genetic evolution,identification of ethnic origin,individual identification and so on.
作者
刘树虎
伊力哈木.乃扎木
王德萍
布帕提玛穆.阿布都克热穆
热比亚木.巴克
多力坤.买买提玉素甫
Shuhu Liu;Nizam Yilihamu;Deping Wang;Rabiyamu Bake;Abdukeram Bupatima;Dolkun Matyusup(College of the Life Sciences and Technology, Xinjiang University, Urumqi 830046, Chin)
出处
《生物技术》
CAS
2018年第2期151-157,共7页
Biotechnology
基金
国家自然科学基金项目("新疆隔离人群的遗传多样性研究及流行病学资源数据库建立"
No.31460285)
中科院上海生命科学研究院计算生物研究所重点实验室开放课题(No.2014KLCB02)
