摘要
为了进一步阐明广藿香中药用活性成分生物合成的分子机制,本研究以海南广藿香幼叶及成熟叶片为材料,采用BGISEQ-500高通量测序平台进行转录组测序,分别获得了63 751 826条和65 949 390条clean reads,平均读长为90 nt。De novo组装后将All-unigene分别注释到Nr、KOG、GO、KEGG、Swiss-Prot、Inter Pro数据库,对每个数据库注释的Unigene数目进行统计,共有162 509条Unigene有对应的功能信息,其中105 430条Unigene被注释到Nr数据库,显示与芝麻有69.87%的相似度;有83 369条Unigene被注释到KOG数据库,根据功能将其分为25类;有12 261条Unigene与GO数据库中的基因具有相似性,将其归为3大类中49个功能组;有79 053条Unigene被注释到KEGG的代谢通路中,分属于124类代谢通路,包括次生代谢物质生物合成、倍半萜和三萜类化合物生物合成、黄酮和黄酮醇生物合成、花青素生物合成等。该研究结果对广藿香药用活性成分生物合成与代谢、关键酶基因克隆以及分子标记开发等研究有一定的帮助。
The aim of this study was to further understand the molecular mechanism of active pharmaceutical ingredients biosynthesis in Pogostemon cablin. In this study, young leaves and mature leaves of P. cablin were selected as materials, and BGISEQ-500 high throughput sequencing platform was used to carry out RNA-seq, the results of which showed that 63 751 826 and 65 949 390 clean reads were obtained, respectively, with the mean length of 90 nt. After De novo assembly, All-unigene were annotated to Nr, KOG, GO, KEGG, Swiss-Prot and InterPro databases. The number of Unigene annotated to each database was calculated. In total, 162 509 Unigene had the corresponding function information, in which 105 430 Unigene were annotated to Nr database, sharing 69.87% similarity with Sesamum indicum. 83 369 unigene were annotated to KOG database, which could be divided into 25 classes according to the function. 12 261 unigene had similarity with genes in GO databse, which divided into 3 categories with a total of 49 functional groups. 79 053 Unigene were annotated to KEGG pathway, F which belonged to 124 metabolic pathways, including biosynthesis of secondary metabolites, sesquiterpenoid and triterpenoid biosynthesis, flavone and flavonol biosynthesis, anthocyanin biosynthesis and so on. These data wouldhelp the study on medicinal active components biosynthesis and metabolism in P. cablin, cloning of the key enzyme gene and development of molecular markers.
作者
陈赫
吴友根
杨豫章
庞真真
张军锋
于靖
Chen He;Wu Yougen;Yang Yuzhang;Pang Zhenzhen;Zhang Junfeng;Yu Jing(College of Tropical Agriculture and Forestry, Hainan University, Haikou, 57022)
出处
《分子植物育种》
CAS
CSCD
北大核心
2018年第7期2139-2154,共16页
Molecular Plant Breeding
基金
国家自然科学基金项目(81360618
81660633)资助
关键词
广藿香
转录组
基因功能
Pogostemon cablin, Transcriptome, Gene function
