摘要
比较了SDS法和Trizol法提取树莓果实总RNA的效果。结果表明:Trizol法提取的RNA经电泳检测,未见条带,说明Trizol法并不适合富含多糖多酚的树莓。SDS方法提取树莓果实总RNA完整性好,28S和18S条带清晰,OD260/OD280值为1.83,在1.7～2.0之间,OD260/OD230值为2.01。结果证明,SDS法提取的总RNA可直接用于分子克隆等分子生物学实验。
SDS and Trizol were compared for extracting total RNA from fruit of raspberry in this paper.The results showed that no bands of RNA isolated by Trizol were found by agarose gel electrophoresis,indicating Trizol method was not suitable for the raspberry rich in polysaccharides and polyphenols.Total RNA isolated by the method of SDS displayed a good integrity,with clear bands of 28S and 18S,OD260/OD280 was 1.83 between 1.7 and 2.0,and OD260/OD230 was 2.01.Reverse transcription results showed that total RNA extracted by the SDS can be directly used for molecular cloning and other molecular biology experiments.
出处
《北方园艺》
CAS
北大核心
2011年第1期136-138,共3页
Northern Horticulture
基金
国家公益性行业农业科研专项基金资助项目(ny-hyzx07-028)
