摘要
针对香蕉组织中多糖、多酚等次生物质含量高的特点,采用改良Trizol法,成功地从香蕉叶片中提取了总RNA。所提取的总RNA A260/A280和A260/A230值分别为1.91和2.10。电泳检测显示28 s、18 s条带完整清晰,以此RNA为模板进行RT-PCR,结果成功得到目标特异条带。试验结果表明:改良Trizol法具有方便快捷、完整性好、受多糖多酚影响较少以及无DNA和蛋白质污染等优点,对具有香蕉叶片类似成分的其他作物的RNA提取具有一定的借鉴意义。
The total RNA was extracted from mature banana leaf which had plenty of polysaccharides and hydroxybezene with improved Trizol method. The electrophoresis pattern showed the bands of 28 s rRNA and lgs rRNA were sharp and complete, A260/A280and A260/A230 values of the extracted RNA were 1.91 and 2.10. The result of RT-PCR with the total RNA was positive. RNA with high quality and high quanity can be extracted with this method from plant materials which have plenty of polysaccharides and hydroxybezene.
出处
《广东农业科学》
CAS
CSCD
北大核心
2009年第5期162-164,共3页
Guangdong Agricultural Sciences
基金
中央级公益性科研院所基本科研业务费专项(SSRI200714)
海南省自然科学基金资助项目(39870532)
