摘要
为得到高质量的谷子种子RNA,本研究以5个不同谷子品种为材料,对RNAiso Plus法、改良RNAiso Plus法、CTAB法提取的RNA进行质量和纯度的比较。结果表明,改良RNAiso Plus法提取的总RNA完整性、纯度、浓度均优于其他2种方法提取的总RNA,5个不同谷子品种和不同萌发时期的谷子均可用改良RNAiso Plus法提取出高质量、完整性好的RNA,所得RNA产物可用于构建cDNA文库和转录组测序等后续试验。本研究为禾谷类植物种子的总RNA提取提供了一定的参考,也为谷子种子后续的分子生物学试验奠定了基础。
In order to obtain high-quality RNA from millet seeds,5 different millet varieties were used as materials,the quality and purity of total RNA extracted with the method of RNAiso Plus,modified RNAiso Plus and CTAB RNA respectively were compared and analyzed.The results showed that the integrity,purity and concentration of total RNA extracted with the modified RNAiso Plus method was better than that extracted with the other two methods.High-quality,well-completed RNA can be obtained from all five millet varieties and also the millet at different germination stagewith the modified RNAiso Plus method.The isolated RNA was suitable for subsequent molecular manipulations,such as cDNA bank construction and transcriptome sequencing.This study lays a foundation for further molecular analysis.At the same time,it provides some reference for total RNA extraction from the seeds of other cereals.
作者
王金荣
许冰霞
尹美强
温银元
WANG Jinrong;XU Bingxia;YIN Meiqiang;WEN Yinyuan(College of Agricultural,Shanxi Agricultural University,Taigu,Shanxi 030801)
出处
《核农学报》
CAS
CSCD
北大核心
2020年第4期729-734,共6页
Journal of Nuclear Agricultural Sciences
基金
山西省重点研发计划(201603D221003-2)
山西农谷建设科研专项项目(SXNGJSKYZX201704)
国家“十三五”谷子高粱产业技术体系项目(GARS-06-13.5-A28)
山西农业大学青年拔尖创新人才培养计划(TYIT201406).
