摘要
目的：以茜草醇提物干预AIA大鼠模型，探讨茜草醇提物对佐剂性关节炎（AIA）大鼠模型肝脏、脾脏损伤及脾脏组织中叉状头／翅膀状螺旋转录因子3（Foxp3）的影响。方法：将72只Wistar大鼠随机分为正常组、模型组、地塞米松（0．125mg·kg^-1）组和茜草醇提物低、中、高剂量组（2．5，5，10g·kg^-1），每组12只。适应性喂养7d后，于其余5组大鼠右后足跖皮下注射弗氏完全佐剂（CFA）0．1mL致炎，复制AIA模型，正常组给予等体积的蒸馏水，连续灌胃给药21d。给药期间，用排水法测定致炎侧大鼠足体积，观察大鼠行为、体质量增长情况。给药21d后，计算大鼠足肿胀度、脏器指数，检测肝脏组织中超氧化物歧化酶（SOD），丙二醛（MDA），髓过氧化物酶（MPO），一氧化氮（NO）水平，苏木素一伊红（HE）染色观察肝脏、脾脏病理组织学变化，免疫组化法检测大鼠脾脏组织内Foxp3蛋白表达情况。结果：与正常组比较，模型组大鼠足肿胀显著升高，脾脏、肝脏脏器指数明显降低，肝脏组织中MDA和MPO含量明显增加，脾脏中Foxp3蛋白水平显著降低（P〈0．05，P〈0．01）。与模型组比较，茜草醇提物中、高剂量组及地塞米松组大鼠足肿胀明显降低（P〈0．05，P〈0．01），但茜草醇提物低剂量组无显著性差异；茜草醇提物低、中、高剂量组大鼠脾脏、肝脏脏器指数显著增加（P〈0．01）；改善肝脏及脾脏组织炎症变化，茜草醇提物低、高剂量组及地塞米松组均可以明显降低MDA和MPO的含量（P〈0．05，P〈0．01），仅茜草醇提物高剂量组及地塞米松组显著升高SOD活性和降低NO的含量（P〈0．01），茜草醇提物中、高剂量组及地塞米松组明显上调脾脏中Foxp3蛋白水平（P〈0．05，P〈0．01）。结论：茜草醇提物具有较好的抗炎作用，表明茜草醇提物可以改善AIA大鼠肝脏及脾
Objective： To investigate the effects of Rubiae Radix et Rhizoma extract （RRE） on liver, spleen injury and the expression of forkhead box P3 （Foxp3） in adjuvant-induced arthritis （AIA） rats. Method：Totally 72 Wistar rats were randomly divided into normal control （Con） group, model （AIA） group, dexamethasone （Dex） （0. 125 mg·kg-1） group and RRE low, medium and high dose groups （2.5, 5, 10 g·kg-1 ） , 12 rats in each group. After 7 days of adaptive feeding, 0. 1 mL Freund＇s complete adjuvant （CFA） solution was injected subcutaneously into the hind paw of each rat to establish AIA models, while the rats in normal control group were injected with an equal volume of distilled water. Drugs were given by intragastric administration for 21 consecutive days. During the administration, the volume of the inflammatory side of the paw was measured by the drainage method, and the behavior and weight gain of the rats were observed. After 21 days of administration, the swelling degree of paw and viscera index were calculated; the levels of superoxide dismutase （SOD） , malondialdehyde （MDA） , myeloperoxidase （MPO） and nitric oxide （NO） in liver tissues were detected ; histopathological changes in liver and spleen tissues were observed by hematoxylin-eosin （HE） staining; and the expression of Foxp3 protein in spleen tissues was detected by immunohistochemistry. Result： As compared with the normal group, the paw swelling was significantly increased; viscera indexes of spleen and liver were significantly decreased; levels of MDA and MPO were significantly increased, and Foxp3 protein expression in spleen tissues was significantly decreased in model group （P 〈0.05, P 〈0.01 ）. As compared with the model group, the paw swelling was significantly reduced in RRE middle and high dose groups and Dex group （P 〈 0.05, P 〈 0. O1 ） , but RRE low dose group showed no significant difference; viscera indexes of spleen and liver were significantly increased in RRE low
作者
沈彩红
刘云
余宪文
宋晓娟
肖宗崇
詹若挺
严萍
SHEN Cai-hong1,2, LIU Yun2, YU Xian-wen2, SONG Xiao-juan1,2, XIAO Zong-chong1,2, ZHAN Ruo-ting1,2, YAN Ping1,2.(1. Research Center of Chinese Herbal Resource Science and Engineering, Guangzhou University of Chinese Medicine; Key Laboratory of Chinese Medicinal Resources from Lingnan, Ministry of Education; Joint Laboratory of Nation Engineering Research Center for the Pharmaceutics of Traditional Chinese Medicines, Guangzhou 510006, China; 2. Guangzhou University of Chinese Medicine, Guangzhou 510006, Chin)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2018年第7期147-153,共7页
Chinese Journal of Experimental Traditional Medical Formulae
基金
广东省科技计划项目(2016ZC0098)
广州中医药大学2017年高水平大学建设面上项目(广中医研[2017]10号)
广东省普通高校创新团队项目(2016KYTD02)
