摘要
目的:建立番茄红素脂质体含量测定的HPLC法。方法:采用RP-HPLC法,色谱柱为Diamonsil C18(150mm×4.6 mm,5μm),流动相为乙腈-二氯甲烷-甲醇,流速为1.0m L/min,柱温为25.0℃,检测波长为472 nm。结果:番茄红素在11.49~34.46μg/m L范围内线性关系良好(r=0.9991);加样回收率为100.40%,RSD%=0.81%。结论:该法专属性强,线性关系良好,快速,准确,简便,适用于番茄红素脂质体的含量测定。
Objective： To establish a method for the content determining of lycopene liposome. Methods： The assay was performed on a Diamonsil C18 （150 mm×4.6 mm, 5μm） column with the mobile phase of acetonitrile- bacetonitrile-methanol, at a flow of 1.0 mL/min; the column temperature were set to 25.0 %, and the UV detection wavelength was 472 nm. Results： The linear ranges of lycopene was 11.49-34.46 μg/mL （r=0.9991）, and the average recoveries were 100.40% （RSD=0.81%）. Conclusion： The method is specificity and rapid with good linear ranges, fast, accurate and easy, which apply to the content determination of lycopene liposome.
作者
王娟
任薇
刘雅娜
韩海霞
包晓玮
朱金芳
WANG Juan, REN Wei, LIU Ya-na, HAN Hai-xia, BAO Xiao-wei, ZHU Jin-fang(College of Food Science and Pharmaceutical Sciences, Xinfiang Agricultural University, Urumqi Xinjiang 830052, Chin)
出处
《中医药导报》
2018年第5期53-54,58,共3页
Guiding Journal of Traditional Chinese Medicine and Pharmacy
基金
新疆维吾尔自治区自然科学基金项目(2015211A026)
