摘要
采用高效液相色谱法建立了同时测定何首乌中大黄酸、大黄素、大黄酚三种蒽醌类化合物的方法,样品采用超声波法提取,色谱柱为Phenomenex C18（250 mm×4.6 mm,5μm）；流动相为甲醇-0.4%醋酸水溶液（80∶20）；流速0.80 mL/min；紫外检测波长254 nm；柱温为35℃.结果：该方法对大黄酸、大黄素和大黄酚分别在0.0001~12.50μg/mL、0.0002~25.00μg/mL、0.00018~22.50μg/mL 范围内线性关系良好,相关系数（r）均大于0.9999,定性检测限（S/N=3）依次是：0.0703 ng/mL、0.0282 ng/mL、0.0871 ng/mL；样品回收率为88.39%~107.2%.结论：本法操作简单快速、定量准确、灵敏度高、成本低,为何首乌中蒽醌类化合物的检测分离及含量测定提供了一个有效的科学方法.
A sensitive and specific high performance liquid chromatography （HPLC）method is developed to determine three anthraquinones,Rhein,Emodin and Chrysophanol,in Polygonum Multiflorum.The three compounds are obtained by ultrasonic extraction,separated on Phenomenex C18 （250 mm×4.6 mm,5μm）with a mixture of methanol -0.4% HAC （volume ration 80：20）as mobile phase at flow rate 0.8 mL/min.The UV detective wavelength is 254 nm,and the column temperature is set at 35 ℃. Result：The calibration curves for Rhein,Emodin and Chrysophanol are linear between 0.0001~12.50μg/mL （r=0.99996）,0.0002~25.00 μg/mL （r=0.99999）and 0.00018~22.50 μg/mL （r=0. 99993）,respectively.The limits of detection （LOD）are 0.073 ng/mL,0.0282 ng/mL and 0.0871 ng/mL,respectively.The recoveries of the three compounds range are between 88.39%~107.2%.The re-sults indicate that the proposed method is simple,rapid,accurate,sensitive,and low cost,thus it pro-vides an effective scientific means for assaying anthraquinones in Polygonum Multifloru.
出处
《四川大学学报（自然科学版）》
CAS
CSCD
北大核心
2014年第2期349-353,共5页
Journal of Sichuan University(Natural Science Edition)
基金
国家科技重大专项(2008ZX07315)
关键词
高效液相色谱
超声提取
蒽醌
何首乌
High performance liquid chromatography
Ultrasonic extraction
Anthraquinone
Polygo-num Multiflorum
