摘要
采用胶束电动毛细管色谱法,建立测定何首乌中二苯乙烯苷、大黄素、大黄酚的新方法,对影响分离的诸因素进行了优化。缓冲液组成为25mmol/L硼砂-40mmol/L十二烷基硫酸钠-10%(体积分数)乙醇,pH值为9.5。在优化的条件下,二苯乙烯苷、大黄素和大黄酚可于20min内分离;线性范围分别为10～1 000,5.2～260,4.5～225mg/L;检出限分别为1.10,0.29,0.43mg/L;平均加标回收率分别为98.8%,98.8%,100.7%。该方法可满足何首乌中二苯乙烯苷、大黄素和大黄酚的测定要求,可作为何首乌药材的质量控制方法。
A new assay is developed by use of micellar electrokinetic capillary chromatography to determine stilbene glycoside,emodin and chrysophanol in radix polygoni muliflori.The factors affecting separation are optimized.The running buffer contains 25 mmol/L borax,40 mmol/L sodium dodecyl sulfate and 10% ethanol,and its pH value is adjusted to 9.5.Under the optimum conditions,the linear dynamic range is 2.0~1 000 mg/L for stilbene glycoside,5.2~260 mg/L for emodin and 4.5~225 mg/L for chrysophanol.The limits of detection are 1.10,0.29 and 0.43 mg/L for stilbene glycoside,emodin and chrysophanol,respectively.The average recoveries are 98.8%,98.8% and 100.7% for stilbene glycoside,emodin and chrysophanol.The assay meets the determination requirement of stilbene glycoside,emodin and chrysophanol in radix polygoni muliflori,and can be applied to quality control of the raw material of radix polygoni muliflori.
出处
《河北科技大学学报》
CAS
北大核心
2011年第5期426-430,481,共6页
Journal of Hebei University of Science and Technology
基金
教育部高等学校博士学科点专项科研基金资助项目(20093227110010)
江苏大学科研基金资助项目(08JDG001)
江苏省高校优势学科建设工程资助项目
关键词
胶束电动毛细管色谱
何首乌
二苯乙烯苷
大黄酚
大黄素
micellar electrokinetic capillary chromatography
radix polygoni muliflori
stilbene glycoside
chrysophanol
emodin
