摘要
目的:建立何首乌复方口服液的质量标准。方法:采用薄层色谱法进行定性鉴别,采用高效液相色谱法测定该制剂中二苯乙烯苷的含量。结果:薄层色谱中供试品与二苯乙烯苷对照品在相应色谱位置上出现相同颜色的斑点,分离良好。二苯乙烯苷在浓度22.5~112.5μg/mL范围内线性良好,平均回收率为99.92%(RSD=0.92%)。精密度、稳定性、重复性试验的RSD均小于2.0%,其平均加样回收率为99.93%(n=6),RSD为0.92%,5批何首乌复方口服液中二苯乙烯苷的平均含量为110.28μg/mL,RSD为0.90%。结论:所建立的定性鉴别和含量测定方法简单可行、重现性好、针对性强,可用于何首乌复方口服液的质量标准控制。
Objective:To improve the quality standard of Polygonum multiflorumoral solution.Methods:Thin layer chromatography was used for qualitative identification,and the content of stilbene glycosides in the preparation was determined by high performance liquid chromatography.Results:In TLC,the same color spots appeared between the test product and the stilbene glycoside reference product on the corresponding chromatographic position,and the separation was good.Stilbene glycoside showed good linearity in the range of 22.5~112.5μg·mL^(-1),and the average recovery was 99.92%,RSD=0.92%.and the RSDof precision,stability and repeatability test were less than 2.0%.The rate was 99.93%(n=6),and the RSD%was 0.92%.The average content of stilbene glycosides in 5 batches of Polygonum multiflorumoral solution was 110.28μg/mL,RSD=0.90%.Conclusion:This method is simple and feasible,reproducible and highly targeted.It can be used to control the quality of Polygonum multiflorumoral solution.
作者
苏穆
王远敏
罗敏
Su Mu;Wang Yuanmin;Luo Min(The Third Affiliated Hospital of Zunyi Medical University,The First People\s Hospital of Zunyi,Zunyi 563000,China)
出处
《亚太传统医药》
2021年第11期78-81,共4页
Asia-Pacific Traditional Medicine
基金
遵义市科技局-遵义市第一人民医院联合科技研发资金项目(遵市科合HZ字(2019)136号,遵市科合HZ字(2019)137号)
