摘要
目的建立牛奶中双酚A(bisphenol A,BPA)的间接竞争化学发光酶免疫分析检测方法。方法通过优化抗原包被浓度、甲醇浓度、酶标二抗的稀释比例、缓冲液的pH、离子强度(以Na^+计)等建立竞争抑制曲线,确定线性范围和检出限,并对牛奶样品进行加标回收实验。结果在最佳实验条件下,半抑制浓度(IC_(50))为3.95 ng/mL,检出限(IC_(10))为0.66 ng/m L,牛奶样品中双酚A加标回收率为95.0%~112.9%,相对标准偏差为0.93%~3.96%。结论该方法具有较高的灵敏度和良好的特异性,适用于牛奶样品中双酚A的测定。
Objective To develop an indirect competitive chemiluminescent enzyme immunoassay （CLEIA） for the detection of bisphenol A in milk samples. Methods The CLEIA conditions including antigen coated concentration, concentration of methanol, concentration of enzyme labeled anti-antibody, pH and ionic strength were optimized to build competitive inhibition curve, determine the linear range and detection limit, and study the recovery of spiked milk samples. Results In the standard curve of the optimized CLEIA, the half maximal inhibitory concentration （ICs0） was 3.95 ng/mL and the limit of detection （LOD） was 0.66 ng/mL. The CLEIA showed good recoveries with spiked milk ranging from 95.0% - 112.9% with the relative standard deviation of 0.93% -3.96%. Conclusion The proposed method has a high sensitivity and good specificity and is suitable for the determination of bisphenol A in milk samples.
作者
郎艺帆
栗凯红
孟玮
刘仁荣
Lang Yifan, Li Kaihong, Meng Wei, Liu Renrong(School of Life Science, Jiangxi Science and Technology Normal University, Nanehang 330000, Chin)
出处
《卫生研究》
CAS
CSCD
北大核心
2018年第2期296-300,共5页
Journal of Hygiene Research
基金
国家自然科学基金(No.81360429)
江西省科技项目(No.GJJ13574
20132BAB214004
KJLD14067)
关键词
双酚A
化学发光酶免疫分析法
牛奶
bisphenol A, chemiluminescent enzyme immunoassay, milk
