摘要
采用毛细管电泳-电导法建立了磺胺嘧啶、磺胺甲嘧啶、磺胺二甲嘧啶3种结构相近磺胺药物的分离检测方法。以2mmol／L磷酸氢二钠-0．6mmol／L磷酸二氢钠为电泳运行液，分离电压＋12kV。上述3种磺胺药物在10min内实现了基线分离。线性检测范围为0．8—100mg／L，检出限为0．2mg／L（S／N=3）。探讨了缓冲溶液的种类、浓度、pH等诸因素对分离检测结果的影响。应用于市售复方磺胺嘧啶片和小儿安散中磺胺药物的含量测定，取得满意结果。
A method for the separation and determination of 3 similar sulfa drugs, i.e. sulfadiazine （SD）, sulfamerazine（SM） and sulfadimidine（ SM2 ）, by capillary zone electrophoresis with conductivity detection was developed. They were baseline separated within 10 min under the following conditions： uncoated fused-silica capillary 50 cm×50μm; running buffer 2.0 mmoL/L Na2HP4 -0. 6 mmol/L NaH2PO4 （pH = 7.6） and separation voltage ＋ 12 kV. The calibration curve shows good linearity in the range of 0. 8 -100 mg/L（ r = 0. 999 6 ） with a detection limit of 0. 2 mg/L（ S/N = 3 ）. The proposed method has been applied to the determination of SD and SM2 in commercial compound sulfonamide tablets and powders with satisfactory results.
出处
《分析测试学报》
CAS
CSCD
北大核心
2006年第3期100-102,共3页
Journal of Instrumental Analysis
基金
广东省自然科学基金资助项目(031589)
中山大学学生科研计划项目(041178)
关键词
毛细管电泳
电导检测
磺胺嘧啶
磺胺甲嘧啶
磺胺二甲嘧啶
Capillary zone electrophoresis
Conductivity detection
Sulfadiazine
Sulfamerazine
Sulfadimidine
