摘要
目的:比较采收于5月、8月及11月贵州产辣蓼水提物的成分差异。方法:以辣蓼水提物收率为指标,以料液比(A)、提取时间(B)和提取次数(C)为考察因素进行正交试验,计算不同考察因素下辣蓼水提物的收率,并比较K值和极差(R)值,以确定辣蓼水提物的提取工艺条件;采用气相色谱质谱联用仪(GC-MS)检测采集于5、8、11月的贵州辣蓼水提物的化学成分的异同。结果:最佳提取工艺为料液比(1∶12)、提取时间100 min、提取次数3次,采集于3个不同月份的辣蓼中共鉴定出115种化学成分,主要含有酯类、烷类、酮类、酸类、萜类和醛类等化合物;采集于5月份的辣蓼中检测出47种化学成分,其中含量较高的有七氟丁酸二十六烷基酯(27.48%)、三十四烷(16.28%)、二十八烷基戊基醚(11.78%)和棕榈酸(4.74%);采集于8月份的辣蓼中检测出53种化学成分,其中含量较高的有3,3',4,4'-四甲氧基二苯乙烯(11.84%)、(S)-2,3-二氢-5-羟基-7-甲氧基-2-苯基-4H-1-苯并吡喃-4-酮(9.55%)、三十四烷(7.70%)和三氟乙酸二十八烷基酯(7.50%);采集于11月份的辣蓼中检测出56种化学成分,其中含量较高的有(E)-4-(3-羟基丙-1-烯-1-基)-2-甲氧基苯酚(9.32%)、2,3-二氢-苯并呋喃(7.31%)、反式对香豆酸(7.22%)和3,3',4,4'-四甲氧基二苯乙烯(6.94%);3个月份采集的辣蓼中共有的化学成分11种。结论:不同季节采集的辣蓼水提物中的化学成分具有明显的差异,硬脂酸和棕榈酸可作为辣蓼脂肪酸类的指纹图谱成分。
Objective： To compare and analyze the difference and similarity of the water extracts from Polygonum hydropiper （PH） collected in different seasons in Guizhou China. Methods： With the wa- ter extract yield as the index and the solid-liquid ratio, the extract time and the extract frequency as factors, the orthogonal test was conducted to determine the extract processing conditions of the water extracts from PH ; GC-MS was adopted to investigate the effects on the change of chemical compositions of the water extracts from PH collected in different seasons. Results： The best extract solid-liquid ratio was 1：12, the extract time was 100 min＇, the extract frequency was three times; 115 chemical compo- sitions of PH collected in 3 months were identified, mainly including esters, alkanes, ketones, acids, terpenoids, aldehydes etc ： 47 chemical compositions of PH collected in May were detected, mainly in- cluding hexacosyl heptafluorobutyrate （27.48 % ）, tetratriaeontane （ 16.28 % ）, octacosyl pentyl ether （ 11.78% ） and hexadecanoic acid （4.74%） ; 53 chemical compositions of PH collected in August were detected, mainly including 3,3＇, 4,4＇-Tetramethoxystilbene （ 11. 84% ） , 4H-1-Benzopyran-4- one, 2,3-dihydro-5-hydroxy-7-methoxy-2-phenyl-, （S）-（9.55% ）, tetratriacontane （7.70%）, octacosyl trifluoroacetate （7.50%） ; 56 chemical compositions of PH collected in November were detecled, mainly including hydroxyprop-I -en-1 -yl） -2-methoxyphenol （ 9.32 ~ ） , henzoturan, 2,3-dihwh＇o-（ 7. 31% ）, p-Coumaric acid, trans（7.22% ）, 3,3＇ ,4,4＇-Tetramethoxystilbene（ 6.94% ） ; There are 11 kinds of common compositions were extracted from PH collected in the three months. Conclusion： There is remarkable difference in the water extracts of PH collected at ditterent seasons. Octadecanoie acid and n-Hexadecanoic acid are recommended as fingerprints of fatty acids in PH,and the fatty acids in PH may be the main active components to promote the proliferation of
出处
《贵州医科大学学报》
CAS
2018年第2期135-143,共9页
Journal of Guizhou Medical University
基金
贵州省科技厅
遵义市科技局
遵义医药高等专科学校联合项目[黔科合LH字(2015)7580号]
遵义市汇川区科技计划项目(遵汇科合201502)
遵义医药高等专科学校博士科研启动经费项目(遵医专科合BS2016002)
关键词
辣蓼
水提物
时间
不同季节
气相
色谱法
Polygonum hydropiper
water extracts
time
different seasons
gas chromatography
