摘要
为明确壳寡糖对干旱胁迫下小麦幼苗叶片中抗坏血酸(AsA)-谷胱甘肽(GSH)循环的影响,以耐旱品种普冰143和干旱敏感品种郑引1号为材料,采用水培试验,研究了喷施100 mg/L壳寡糖溶液对20%PEG模拟干旱胁迫下小麦幼苗叶片活性氧累积、膜脂过氧化水平、AsA-GSH循环关键酶活性以及中间代谢物含量的影响。结果显示,与单一PEG胁迫处理相比,喷施壳寡糖可明显提高PEG胁迫下2种小麦幼苗叶片中的抗坏血酸过氧化物酶(APX)、脱氢抗坏血酸还原酶(DHAR)、单脱氢抗坏血酸还原酶(MDHAR)和谷胱甘肽还原酶(GR)活性以及AsA和GSH含量,维持较高的AsA/DHA(脱氢抗坏血酸)和GSH/GSSG(氧化型谷胱甘肽)比值,同时明显降低超氧阴离子(O-2·)和丙二醛(MDA)含量。表明,在20%PEG模拟干旱胁迫下,喷施100 mg/L的壳寡糖溶液能明显降低小麦叶片中的活性氧含量和膜脂过氧化程度,维持AsA-GSH循环高效运转,提升小麦的抗旱能力。
In order to investigate the effects of chitooligosaccharide on the ascorbate( AsA)-glutathione( GSH) cycle in seedlings of wheat( Triticum aestivumL.) under drought stress,a hydroponic experiment was performed to research the effects of foliar spraying of 100 mg/L chitooligosaccharide solution on the reactive oxygen species accumulation,membrane lipid peroxidation,key enzymes activity and metabolites contents of AsA-GSH cycle in leaves of two wheat cultivars Pubing 143( tolerant to drought stress) and Zhengyin 1( sensitive to drought stress) under 20% PEG stress. The results showed that foliar spraying of100 mg/L chitooligosaccharide solution significantly promoted the activities of ascorbate peroxidase( APX),dehydroascorbate reductase( DHAR),monodehydroascorbate reductase( MDHAR) and glutathione reductase( GR),the contents of GSH and AsA,and the ratios of GSH/GSSG( L-glutathione oxidized)and AsA/DHA( dehydroascorbic acid),and obviously decreased the contents of superoxide anion( O-2·) and malondialdehyde( MDA),compared to PEG stress alone. These findings indicated that foliar spraying of 100 mg/L chitooligosaccharide solution could effectively enhance the wheat resistance to drought stress by maintaining the high efficiency metabolism of AsA-GSH cycle and decreasing the reactive oxygen speciesaccumulation and membrane lipid peroxidation in leaves.
出处
《河南农业科学》
CSCD
北大核心
2017年第12期8-12,58,共6页
Journal of Henan Agricultural Sciences
基金
太原工业学院院级青年基金项目(2016LQ15)
